ovalbumin and Carcinoma--Ehrlich-Tumor

Cancer is the leading cause of mortality worldwide. Analysis of epidemiological data has revealed a negative relationship between allergic conditions and cancer incidence. This study addresses the effects of chronic antigen ingestion by sensitized mice (allergy) on Ehrlich tumor growth in mouse footpad. Mice were sensitized (allergic) or not (sham) with ovalbumin and challenged orally with egg white solution. After one week of oral challenge, all mice were inoculated with experimental Ehrlich tumor (EET) cells in the footpad, and tumor growth was evaluated for 21 days. A decrease in tumor growth occurred, as assessed by paw thickness in the allergic group, which was associated with smaller areas of necrosis, reduced infiltration of neutrophils, and reduced levels of IFN-gamma, IL-4, and IL-10. Although, the tumor proliferation rate was similar in both groups, an increase in apoptosis occurred in allergic mice. In conclusion, analysis of the data obtained allows us to suggest that a concomitant allergic condition would reduce tumor progression through increased tumor cell apoptosis, accompanied by reduced areas of necrosis at the tumor site. Indeed, such findings suggested a possible mechanism for the reduced cancer incidence observed in allergic individuals.

Topics: Animals; Apoptosis; Carcinoma, Ehrlich Tumor; Cell Count; Cytokines; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Female; Food Hypersensitivity; Foot; Immunoglobulin E; Immunoglobulin G; In Situ Hybridization; Mice; Mice, Inbred BALB C; Neutrophils; Ovalbumin; Peroxidase; Xenograft Model Antitumor Assays

Pharmacological studies with an aqueous extract obtained from leaves of Capraria biflora showed potent cytotoxic, analgesic, antimicrobial and anti-inflammatory activities. It has been demonstrated that biflorin possesses an in vitro cytotoxic activity against tumor cells. The in vivo antitumor activity of biflorin was evaluated on two mouse models, sarcoma 180 and Ehrlich carcinoma. Biflorin was active against both tumors with a very similar profile. In addition, biflorin was also able to increase the response elicited by 5-FU in mice inoculated with both tumors. The results showed a decrease in Ki67 staining in tumor cells from treated-animals when compared with non-treated groups, which suggests an inhibition of tumor proliferation rate. Histopathological analysis from kidneys and liver showed that biflorin possessed weak and reversible toxic effects. It was also demonstrated that biflorin acts as an immunoadjuvant agent, rising the production of ovalbumin-specific antibodies and inducing a discreet increase of the white pulp and nest of megakaryocytic in spleen of treated mice, which can be related to its antitumor properties.

Topics: Adjuvants, Immunologic; Animals; Antimetabolites, Antineoplastic; Antineoplastic Agents, Phytogenic; Carcinoma, Ehrlich Tumor; Female; Fluorouracil; Immunohistochemistry; Indicators and Reagents; Ki-67 Antigen; Mice; Naphthoquinones; Neoplasm Transplantation; Ovalbumin; Sarcoma 180; Scrophulariaceae

Topics: Animals; Body Water; Brain Chemistry; Carcinoma, Ehrlich Tumor; Electromagnetic Fields; Magnetic Resonance Spectroscopy; Mice; Muscles; Ovalbumin; Protons

Dibrommercuryfluoresceine (DBMF) reacts stoichiometrically and quantitatively with the thiol group of cysteine, glutathione and thioglycolic acid respectively, at pH 7.0. Polarographical and spectrometrical titrations clearly show that in the spectra of the investigated mercaptides the wave length of the first absorption maximum of DMBF (507 nm) remains unchanged but the molar extinction coefficient increases by approximately 20%. Serum albumin, ovalbumin, beta-lactoglobulin and glyceraldehydephosphatedihydrogenase, after incubation with DBMF, form adducts with the dye from which the pure mercaptide complexes were separated by means of column chromatogrphy. These complexes were separated by means of column chromatography. These complexes show a bathochromic shift (520 nm) of the dye band which is decreased now by 50%. The molar extinction coefficient epsilon 520 has been determined from 32,000 to 33,850. On the basis of these values SH-contents of the four proteins were obtained which are in good accordance with data previously published in the literature. No selective reaction, f.i. with more accessible or/and reactive SH-groups was observed. After 30 min incubation with DBMF and washing with isotonic phosphate buffer, native animal tumor cells show in the main absorption band the bathochromically shifted dye maximum. A first temptative estimation of the protein SH-groups yielded 1.7-2.1 X 10(-14) mole SH/single cell. This result lies between the SH-content determined microspectrometrically on cells stained with DDD-Fast Blue B (1.1-1.55 X 10(-14)) and macroscopically on cell homogenates with DTNB (3.1 X 10(-14)). Up to now, no certain information can be given whether or to what extent unspecific absorption effects possibly might be involved in the data obtained with DBMF treated cells, but interaction with nucleic acids can be excluded with certainty on the basis of relevant model experiments.

Topics: Animals; Carcinoma, Ehrlich Tumor; Fluoresceins; Glyceraldehyde-3-Phosphate Dehydrogenases; Lactoglobulins; Merbromin; Mice; Ovalbumin; Polarography; Sarcoma 180; Serum Albumin; Spectrum Analysis; Sulfhydryl Compounds

The effect of various tRNAs on protein synthesis was investigated using a tRNA-dependent cell-free system from Ehrlich ascites cells. Ascites cell tRNA and rabbit liver tRNA were found to promote efficient translation of globin mRNA, oviduct mRNA, and encephalomycarditis (EMC) viral RNA. In contrast, reticulocyte tRNA participated efficiently only in the translation of globin mRNA; the translation of oviduct mRNA AND EMC viral RNA in the presence of reticulocyte tRNA resulted in the synthesis of relatively few large mature proteins and the accumulation of discrete, smaller polypeptides. These results suggest that isoaccepting tRNA species required for the synthesis of ovalbumin and EMC viral protein (but not hemoglobin) are probably functionally absent in reticulocyte tRNA, causing a premature, nonrandom termination of synthesis of these proteins. This provides preliminary evidence that variations in tRNA populations, frequently observed between different cell types, are large enough to define and perhaps regulate the proteins that the cell is capable of synthesizing.

Topics: Animals; Carcinoma, Ehrlich Tumor; Chickens; Electrophoresis, Disc; Encephalomyocarditis virus; Female; Liver; Ovalbumin; Oviducts; Protein Biosynthesis; Rabbits; Reticulocytes; RNA, Messenger; RNA, Transfer; RNA, Viral

Topics: Animals; Carcinoma, Ehrlich Tumor; Cell-Free System; Chickens; Estradiol; Female; Ovalbumin; Protein Biosynthesis; RNA, Messenger; RNA, Transfer

Glucose solution containing wheat straw hemicellulose-B and ovoglycopeptide (W-O-G) has no effect on Ehrlich ascites tumors but completely regresses Ehrlich solid tumors. The carcinostatic effect of this mixture is lost or decreases markedly whenever one of the three substances is lacking. For this glucose solution to possess a carcinostatic effect it is necessary that this hemicellulose-B of W-O-G is the hydrolysate of the extract with 20% NaOH from the residue obtained after extraction of wheat straw with 10% NaOH.

Topics: Animals; Carcinoma, Ehrlich Tumor; Drug Therapy, Combination; Female; Glucose; Glycopeptides; Male; Mice; Mice, Inbred ICR; Neoplasm Transplantation; Ovalbumin; Polysaccharides; Transplantation, Homologous; Triticum

Topics: Animals; Carcinoma, Ehrlich Tumor; Cell-Free System; Guanine Nucleotides; Kinetics; Mice; Ovalbumin; Protein Biosynthesis; RNA, Messenger; Seeds; Species Specificity; Triticum

An antimitotic factor for mouse tumor cells was isolated from the supernatant fluids of antigen-stimulated ovalbumin-immune mouse spleen cells. A similar antimitotic factor was obtained from the supernatant fluids of phytohemagglutinin-stimulated non-immune spleen cells. The inhibitor prevented the multiplication of mouse L929 fibroblast cells in vitro and the in vivo proliferation of Ehrlich ascites tumor cells. Inhibition was not due to cytotoxic effects and the antimitotic effects were reversible. The antimitotic activity may be species-specific since the purified factor obtained from mouse spleen cells had no effect on human or monkey cell lines. This factor appears to be the same as the T lymphocyte-dependent suppressor for antibody production that we described previously. Several implications for the production of the suppressor in response to tumor cells that may be beneficial or harmful to the host are discussed.

Topics: Animals; Antibody-Producing Cells; Carcinoma, Ehrlich Tumor; Centrifugation, Density Gradient; Chick Embryo; Fibroblasts; Haplorhini; Humans; Immunosuppressive Agents; In Vitro Techniques; Injections, Intraperitoneal; Lymphocytes; Male; Mice; Mice, Inbred Strains; Neoplasms, Experimental; Ovalbumin; Species Specificity

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Collagen; Connective Tissue; Glycosaminoglycans; Hexosamines; Hydrocortisone; Hydroxyproline; Injections, Intraperitoneal; Injections, Subcutaneous; Neoplasm Transplantation; Ovalbumin; Uronic Acids

Topics: Adsorption; Aminobenzoates; Animals; Antibodies; Antibody Formation; Ascitic Fluid; Azo Compounds; Bence Jones Protein; Carcinoma, Ehrlich Tumor; Cattle; Fibrinogen; Freund's Adjuvant; gamma-Globulins; Haptens; Hemocyanins; Immune Sera; Immunoelectrophoresis; Iodine Isotopes; Mice; Mollusca; Ovalbumin; Precipitin Tests; Rabbits; Serum Albumin, Bovine

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Collagen; Fibrinogen; Hydroxyproline; Male; Mice; Muramidase; Ovalbumin