ovalbumin and Anemia--Hemolytic--Autoimmune

In the last ninety years there were many attempts to induce immune haemolytic anaemia (IHA) in laboratory animals. The techniques employed were injections of modified autologous or incompatible erythrocytes as well as hyperimmunization with material of high antigen activity (e.g. egg albumin). Serious temporary IHA could be induced occasionally. The main pathogenetic mechanism in this immune reaction was related to the antigen. Another model used was the so called graft-host-reaction in which IHA is based on antibody formation of transplanted immunocompetent cells towards the host erythrocytes. So far, the best animal model is the spontaneous autoimmune haemolytic anaemia in NZB-mice. However this model has not been completely evaluated. In these animals AIHA is mediated by a genetically prescribed immunoregulatory defect which may be located at the level of T-lymphocytes.

Topics: Anemia, Hemolytic, Autoimmune; Animals; Autoantibodies; B-Lymphocytes; Disease Models, Animal; Erythrocytes; Graft vs Host Reaction; Humans; Immunization; Mice; Mice, Inbred NZB; Ovalbumin; T-Lymphocytes

Topics: Adrenocorticotropic Hormone; Anemia, Hemolytic, Autoimmune; Animals; Coombs Test; Disease Models, Animal; Erythrocyte Count; Erythrocytes; Humans; Immunoglobulin G; Immunoglobulins; Injections, Intravenous; Methods; Ovalbumin; Prednisolone; Rabbits; Spleen; Splenectomy

Topics: Aleutian Mink Disease; Anemia, Hemolytic, Autoimmune; Animals; Antibodies, Antinuclear; Antigen-Antibody Reactions; Collagen Diseases; Disease Models, Animal; Graft vs Host Reaction; Lupus Erythematosus, Systemic; Lymph Nodes; Mice; Ovalbumin; Rabbits; Spleen; Transplantation, Homologous

Young adult mice (30 to 43 days old) of autoimmune NZB and BIW F(1) strains failed to develop immunologic tolerance when treated with ultracentrifuged bovine gamma globulin and then challenged 12 days later. By contrast, weanling NZB and B/ W F(1) mice (18 to 25 days) as well as weanling C3H mice (16 to 19 days) became tolerant and had no serum antibody 12 days after challenge. The C3H mice remained unresponsive, but NZB and B! W F(1) mice produced antibody between days 27 and 41. The rapid loss of previously established tolerance to foreign antigens could have its parallel in the loss of tolerance to autoantigens with subsequent development of lupus nephritis and Coombs' positive hemolytic anemia in these animals.

Topics: Anemia, Hemolytic, Autoimmune; Animals; Animals, Newborn; Antibody Formation; Autoantibodies; Cattle; gamma-Globulins; Immune Tolerance; Lupus Erythematosus, Systemic; Mice; Nephritis; Ovalbumin