ovalbumin and Agammaglobulinemia

Following allergen challenge of sensitized mice, neutrophils are the first inflammatory cells found in bronchoalveolar lavage (BAL) fluid. To determine the underlying mechanism for their accumulation, mice were sensitized to OVA on days 0 and 14, and received, on day 28, a single intranasal challenge (s.i.n.) with either OVA or ragweed. Eight hours after the s.i.n., BAL fluid was obtained. BALB/c mice sensitized and challenged with OVA showed significantly higher total cell counts and numbers of neutrophils in BAL fluid compared to the OVA-sensitized and ragweed-challenged or nonsensitized mice. Levels of neutrophil chemokines in BAL fluid supernatants were markedly elevated in the sensitized and OVA-challenged mice; Fc epsilon RI-deficient mice showed comparable numbers of neutrophils and neutrophil chemokines in BAL fluid after s.i.n. But in sensitized mice lacking the Fc common gamma-chain and B cell-deficient mice, the number of neutrophils and levels of neutrophil chemokines in BAL fluid were significantly lower. Further, mice lacking the FcgammaRIII did not develop this early neutrophil influx. Neutrophil infiltration could be induced in naive mice following intranasal instillation of allergen combined with allergen-specific IgG1. In addition, macrophages from sensitized mice were stimulated with allergen and activated to produce neutrophil chemokines. These results demonstrate that neutrophil influx after allergen challenge requires prior sensitization, is allergen-specific, is mediated through FcgammaRIII, and is dependent on the presence of Ab.

Topics: Administration, Intranasal; Agammaglobulinemia; Allergens; Ambrosia; Animals; Antibody Specificity; B-Lymphocytes; Bronchoalveolar Lavage Fluid; Cells, Cultured; Chemokines; Epitopes; Female; Immunization; Immunoglobulin E; Immunoglobulin G; Lymphopenia; Macrophages, Alveolar; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Neutrophil Infiltration; Neutrophils; Ovalbumin; Receptors, IgG

Despite the absence of B lymphocytes, peripheral blood lymphocytes (PBL) from four of five patients with congenital agammaglobulinemia (cAgamma) generated a specific hemolytic plaque-forming cell (HcPFC) response in vitro to sheep red blood cells and ovalbumin. The kinetics, antigenic, and cellular requirements were similar to normals, but significantly less HcPFC were found in patient cultures. Normal but not patient HcPFC-precursor cells were inactivated by treatment with anti-mu antisera whereas generated HcPFC in both controls and patients were sensitive to treatment with anti-mu. Pokeweed mitogen (PWM) and dextran sulfate (DXS) enhanced the HcPFC-response of normal PBL; cAgamma-cells were unresponsive to DxS and, in the presence of PWM, the development of HcPFC was inhibited. These findings indicate the presence of B lymphocyte precursors in the majority of patients with cAgamma investigated.

Topics: Adolescent; Agammaglobulinemia; Animals; B-Lymphocytes; Cell Differentiation; Child; Child, Preschool; Dextrans; Erythrocytes; Hemolytic Plaque Technique; Humans; Lymphocyte Activation; Lymphocyte Cooperation; Male; Mitogens; Ovalbumin; Sheep

Topics: Absorption; Agammaglobulinemia; Animals; Complement System Proteins; Escherichia coli; Hot Temperature; Humans; Immune Sera; Immunodiffusion; Opsonin Proteins; Ovalbumin; Rabbits; Staphylococcus

Topics: Agammaglobulinemia; Animals; Freund's Adjuvant; gamma-Globulins; Immune Sera; Immunization; Immunoelectrophoresis; Injections, Intraperitoneal; Mice; Multiple Myeloma; Oils; Ovalbumin; Plasmacytoma; Research; Vaccination