osteum and Hemolysis

The hemolytic activity of sodium oleate, a high lytic agent, was investigated in different surfactant solutions and lipid emulsion formulations. A new explanation of the protective function of these systems is proposed. It was found that the hemolytic activity of the lytic agent was greatly decreased in solutions and/or dispersions with the surfactant Cremophor EL, Solutol H16 and phospholipids, which can usually build a micellar or liposomal structure. In the case of F68, where the micelle formation is still controversial, the hemolytic activity of the lytic agent was practically not affected and complete hemolysis was observed. In contrast to this, all emulsion formulations, independent of the emulsifier type, showed a stable erythrocyte behavior. Additionally, in the case of lipid emulsions only, a larger amount of the lytic agent could be added without any remarkable increase in the hemolytic activity. As an explanation for these effects it is proposed that the lytic agent is either incorporated into the lipophilic core or intercalates between the emulsifier molecules at the interface. This decreases the direct contact of the lytic agent with the erythrocyte membrane. As a result, the erythrocytes will effectively be protected from hemolytic damage, which can otherwise be induced by such substances.

Topics: Adult; Emulsions; Erythrocytes; Glycerol; Hemolysis; Humans; In Vitro Techniques; Liposomes; Male; Micelles; Oleic Acid; Phospholipids; Polyethylene Glycols; Stearic Acids; Surface-Active Agents

Topics: Erythrocytes; Hemolysis; Humans; Hyperlipidemias; Oleic Acid

Topics: Erythrocytes; Hemolysis; Humans; Hyperlipidemias; Oleic Acid; Oleic Acids; Plasma

Sodium oleate reacts progressively with human red cells at pH 7. By progressive is meant a reaction which is not adequately described as reversible or irreversible; such reactions cannot be stopped once they are under way, and are probably associated with a more or less stable "internal" lysin phase at the cell surfaces. The uptake of the lysin and the effect of dilution on the uptake can be studied by converting sodium oleate into the radioactive form, sodium oleate-I(131). The uptake is a parabolic function of the lysin initially present in the system, and the effect of a tenfold dilution of systems in which red cells have remained in contact with the lysin for 2 minutes is to reduce the lysin taken up at the cell surfaces twofold. The lysin rapidly forms a relatively stable layer at the cell interfaces, and this layer is little affected by the dilution of the system as a whole.

Topics: Cell Death; Cell Membrane; Erythrocytes; Hemolysin Proteins; Hemolysis; Oleic Acid; Oleic Acids