osteoprotegerin and Jaw--Edentulous

The aim of this split-mouth, randomized, double-blind, controlled clinical trial was to evaluate the influence of different insertion torque values for dental implants on bone- and angiogenesis-related marker profiles. Eighteen edentulous patients received dental implants and fixed complete-arch mandibular prostheses. The implants (n=36) were assigned randomly to two groups: reduced torque (n=18), with insertion torque <30Ncm; and conventional torque (n=18), with insertion torque ≥30Ncm. Levels of vascular endothelial growth factor (VEGF), placental growth factor, bone morphogenetic protein 9 (BMP-9), periostin, osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRAP) in the peri-implant fluid were quantified at 7, 14, 30, and 120days after implant placement. Inter-group comparisons showed that VEGF and OPG levels were higher in the low-level torque group than in the conventional torque group on days 7 and 30, respectively (P<0.05). BMP-9 and periostin levels were higher in the conventional group than in the low-level torque group on day 120, and TRAP was up-regulated around implants inserted with conventional torque when compared to those inserted with lower-level torque at all time points evaluated (P<0.05). In conclusion, the use of different levels of torque for implantation of immediately loaded implants significantly influenced the levels of bone- and angiogenesis-related markers during early peri-implant repair.

Topics: Adult; Aged; Biomarkers; Cell Adhesion Molecules; Double-Blind Method; Female; Growth Differentiation Factor 2; Humans; Immediate Dental Implant Loading; Jaw, Edentulous; Male; Middle Aged; Osteoprotegerin; Placenta Growth Factor; Prospective Studies; Tartrate-Resistant Acid Phosphatase; Torque; Vascular Endothelial Growth Factor A

This 3-year prospective randomized controlled trial compared the clinical, microbiological and biochemical outcome of minimally (Turned, Tur) and moderately rough (TiUnite(®) , TiU) implant surfaces in a split-mouth design.. The study population included 14 subjects: nine fully edentulous and five partially edentulous subjects with a history of periodontitis. Implants (n = 78, 39 Tur and 39 TiU) were installed randomly in each patient. Peri-implant clinical parameters and intra-oral radiographs were recorded after 3 years of loading. Subgingival plaque and peri-implant crevicular fluid samples were collected and analysed using culture and quantitative polymerase chain reaction for the biofilm, and enzyme-linked immunosorbent assay for the concentration of osteoprotegerin and receptor activator of nuclear factor kappa-B ligand, respectively.. No statistically significant differences in clinical, microbiological and biochemical parameters could be observed when comparing the Tur and TiU implant surfaces.. After 3 years of loading, in periodontitis susceptible patients, the moderately rough, TiU implants demonstrated a similar clinical outcome compared with the smoother, turned implants. Longer follow-up and studies using different implant types are needed to confirm the statement that minimally and moderately rough implant surfaces perform similar, both from a clinical and from a microbiological point of view.

Topics: Aged; Alveolar Bone Loss; Analysis of Variance; Chronic Periodontitis; Coated Materials, Biocompatible; Dental Implants; Dental Prosthesis Design; Dental Prosthesis, Implant-Supported; Female; Follow-Up Studies; Gingival Crevicular Fluid; Gingival Recession; Humans; Jaw, Edentulous; Jaw, Edentulous, Partially; Male; Middle Aged; Osteoprotegerin; Oxides; Periodontal Index; Pilot Projects; Prospective Studies; RANK Ligand; Surface Properties

This study assessed gene expression by quantitative polymerase chain reaction of inflammatory- [interleukin (IL)-12, tumor necrosis factor-alpha (TNF-alpha), IL-4, and IL-10] and osteoclastogenesis-related factors [receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG)] in sites exhibiting different severities of peri-implant disease.. Peri-implant soft tissue biopsies (n=48) were harvested from healthy implant (HI), mucositis (MC), initial peri-implantitis (IP) and severe peri-implantitis (SP) sites.. IL-12 and TNF-alpha mRNA levels were higher in SP, followed by IP and MC (P <0.05). IL-4 was higher in HI, followed by MC, SP and IP (P <0.05). IL-10 was the lowest in HI, while no differences were detected among the diseased groups (P>0.05). OPG mRNA levels were higher in HI, followed by IP, SP and MC, whereas RANKL was increased as the peri-implantitis severity increased (P<0.05). The highest OPG/RANKL ratio was observed in HI and the lowest in SP (P<0.01).. These findings suggest that expressions of inflammatory- and osteoclastogenesis-related factors may play an important role in the onset and severity of the peri-implant diseases.

Topics: Adult; Analysis of Variance; Cell Differentiation; Cytokines; Dental Implants; Female; Gene Expression Regulation; Humans; Interleukin-10; Interleukin-12; Interleukin-4; Jaw, Edentulous; Male; Middle Aged; Mucositis; Osteoclasts; Osteoprotegerin; Periodontitis; RANK Ligand; Reference Values; RNA, Messenger; Severity of Illness Index; Tumor Necrosis Factor-alpha