orobol and Melanoma

Effects of sophoricoside and its analogs on proinflammatory cytokines have been investigated. Sophoricoside, genistein and orobol exhibited inhibitory effects on IL-5, IL-3, GM-CSF and IL-6 bioactivities. Genistin showed inhibitory effects on IL-5 and IL-3 bioactivities, but did not inhibit GM-CSF and IL-6 bioactivities. None of the sophoricoside analogs showed inhibitory effects on both IL-1beta and TNF-alpha bioactivities. Among the compounds, sophoricoside exhibited the highest inhibitory effects on IL-5, IL-3 and IL-6 bioactivities with IC50 values of 1.9 microM, 6.9 microM and 6.0 microM, respectively and orobol did show on GM-CSF bioactivity with an IC50 value of 18.0 microM. The result would provide an additional mechanism by which the compounds exert immunosuppressive and anti-inflammatory effects.

Topics: Animals; B-Lymphocytes; Benzopyrans; Cell Division; Cell Line; Cytokines; Flavonoids; Genistein; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Interleukin-1; Interleukin-3; Interleukin-5; Interleukin-6; Isoflavones; Leukemia, Erythroblastic, Acute; Melanoma; Mice; Recombinant Proteins; Structure-Activity Relationship; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha