orabase and Colorectal-Neoplasms

To assess by prospective randomized controlled trial the feasibility and efficacy of using a bioresorbable hyaluronic acid/carboxymethylcellulose membrane (HA membrane) to prevent abdominal and perihepatic adhesions in metastatic colorectal cancer patients requiring 2-stage hepatectomy.. Two-stage hepatectomy offers the possibility of long-term survival to selected patients whose liver metastases cannot be removed in a single procedure. However, the second operation is made more difficult by adhesions arising from the first. HA membrane reduces adhesions in gynecologic and abdominal surgery but this is the first trial in hepatectomy.. Fifty-four candidates for 2-stage hepatectomy were randomized at the end of the first procedure to implantation of HA membrane (n = 41) or standard management (n = 13). Thirty patients from the membrane arm and 11 well-matched controls underwent the planned second hepatectomy.. Positioning of the HA membranes was feasible in all but one patient and did not increase complications associated with the first hepatectomy. At second hepatectomy, patients in the HA membrane arm required 33% less time than controls to achieve complete liver mobilization (median: 50 vs 75 minutes; primary endpoint). The risk of extensive adhesions was reduced in the HA membrane group (31% had grade 3-4 adhesions vs 55% in controls), as was adhesion severity (17% thick and hypervascular adhesions vs 46%). The proportion of patients with complications at second hepatectomy was higher in the control group (55% vs 23% in the HA membrane group, P = 0.07).. Use of 4 HA membranes at the end of first hepatectomy reduced the extent and severity of adhesions and facilitated the second hepatectomy in patients with liver metastases who required a 2-stage hepatectomy. A larger study to confirm these findings is warranted. (NCT01262417).

Topics: Absorbable Implants; Adult; Aged; Carboxymethylcellulose Sodium; Colorectal Neoplasms; Female; Hepatectomy; Humans; Hyaluronic Acid; Liver Neoplasms; Male; Membranes, Artificial; Middle Aged; Prospective Studies; Reoperation; Survival Rate; Tissue Adhesions; Treatment Outcome

An antimicrobial dressing containing ionic silver was found effective in reducing surgical-site infection in a preliminary study of colorectal cancer elective surgery. We decided to test this finding in a randomized, double-blind trial.. Adults undergoing elective colorectal cancer surgery at two university-affiliated hospitals were randomly assigned to have the surgical incision dressed with Aquacel Ag Hydrofiber dressing or a common dressing. To blind the patient and the nursing and medical staff to the nature of the dressing used, scrub nurses covered Aquacel Ag Hydrofiber with a common wound dressing in the experimental arm, whereas a double common dressing was applied to patients of control group. The primary end-point of the study was the occurrence of any surgical-site infection within 30 days of surgery.. A total of 112 patients (58 in the experimental arm and 54 in the control group) qualified for primary end-point analysis. The characteristics of the patient population and their surgical procedures were similar. The overall rate of surgical-site infection was lower in the experimental group (11.1% center 1, 17.5% center 2; overall 15.5%) than in controls (14.3% center 1, 24.2% center 2, overall 20.4%), but the observed difference was not statistically significant (P = 0.451), even with respect to surgical-site infection grade 1 (superficial) versus grades 2 and 3, or grade 1 and 2 versus grade 3.. This randomized trial did not confirm a statistically significant superiority of Aquacel Ag Hydrofiber dressing in reducing surgical-site infection after elective colorectal cancer surgery.. Clinicaltrials.gov: NCT00981110.

Topics: Adolescent; Adult; Aged; Anti-Infective Agents; Bandages; Carboxymethylcellulose Sodium; Colorectal Neoplasms; Double-Blind Method; Drug Carriers; Female; Follow-Up Studies; Humans; Male; Middle Aged; Neoplasm Grading; Prognosis; Prospective Studies; Silver; Surgical Wound Infection; Wound Healing; Young Adult

Inflammation is a well-characterized critical driver of gastrointestinal cancers. Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota. Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development. Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas. In APC

Topics: Adenoma; Animals; Carboxymethylcellulose Sodium; Carcinogenesis; Cell Proliferation; Colorectal Neoplasms; Diet; Emulsifying Agents; Female; Food Additives; Gastrointestinal Microbiome; Gastrointestinal Tract; Inflammation; Intestinal Mucosa; Intestines; Male; Mice; Mice, Inbred C57BL; Polysorbates

Poly(lactide-co-glycolide) (PLGA) colloidal particles stabilized by complexes of two oppositely charged polysaccharides, chitosan (cationic, CS) and sodium carboxymethylcellulose (anionic, NaCMC), were fabricated. Dichloromethane containing dissolved PLGA was first emulsified in an aqueous phase containing mixtures of CS and NaCMC. Evaporation of dichloromethane from the resulting emulsion led to CS/NaCMC-covered-PLGA particles. CS and NaCMC contents affected the short-term stability of PLGA particles and also their intrinsic characteristics. The particles displayed pH-dependent characteristic. Zeta potential varied from +54 to -50 mV when pH was varied from 3 to 10. CS/NaCMC-covered-PLGA particles showed colloidal stability, over a wider pH range as compared to CS-covered-PLGA particles. Curcumin, a model hydrophobic drug, was encapsulated into the particles up to 10 wt% of PLGA. The CS/NaCMC-covered-PLGA particles loaded with curcumin showed delayed release in mildly acidic conditions and faster release in neutral and basic conditions. Cytotoxicity experiments were carried out with human colorectal carcinoma cells.

Topics: Antineoplastic Agents; Carboxymethylcellulose Sodium; Cell Proliferation; Chitosan; Colorectal Neoplasms; Curcumin; Drug Carriers; Drug Delivery Systems; Drug Screening Assays, Antitumor; HCT116 Cells; Humans; Molecular Structure; Particle Size; Polylactic Acid-Polyglycolic Acid Copolymer; Surface Tension; Tumor Cells, Cultured

Due to the decrease of the drugs side effects in controlled drug delivery systems, today's many research has been focused on designing the new controlled drug delivery systems. The main aim of the present work was the improving of the layered double hydroxide (LDH) properties as a drug delivery system through the encapsulation with carboxymethyl cellulose (CMC). In the first section, 5-fluorouracil (5-Fu) as a colon anticancer drug was loaded in LDH(Zn/Al) (about 87%). The FT-IR, SEM, and XRD analysis were used to probe the successful synthesis of LDH(Zn/Al) and 5-Fu loading in it. In the following, the nanohybrid encapsulated with CMC, through the crosslinking of the LDH(Zn/Al)-5-Fu and CMC mixture with Fe(III) as a physical crosslinker and CMC/LDH(Zn/Al)-5-Fu hydrogel beads were formed. From comparing of the 5-Fu release from the LDH(Zn/Al)-5-Fu and CMC/LDH(Zn/Al)-5-Fu hydrogel beads, it was found that the use of CMC as a capsule induce the controlled and sustained drug release behavior to the system. MTT assay approved the biocompatibility of the CMC/LDH(Zn/Al)-5-Fu hydrogel beads. With considering the swelling, drug loading, drug-releasing, and MTT assay results, the prepared CMC/LDH(Zn/Al)-5-Fu hydrogel beads system could be proposed as a potentially safe oral delivery system for colon cancer therapy.

Topics: Administration, Oral; Capsules; Carboxymethylcellulose Sodium; Colorectal Neoplasms; Drug Carriers; Drug Liberation; Fluorouracil; Green Chemistry Technology; Hydroxides; Mechanical Phenomena; Nanostructures

Poor aqueous solubility of chemotherapeutics such as SN38 (7-ethyl-10-hydroxycamptothecin) and the associated systemic adverse effects are serious limitations of their clinical use. To improve the drug delivery efficiency of such compounds, they were covalently conjugated to hydrophilic macromolecular carriers that specifically deliver the drug moiety to the tumour cells. In the current study, we developed a PEGylated acetylated carboxymethylcellulose conjugate of SN38 which was covalently attached to an aptamer against a cancer stem cell marker, CD133. Then, the designed nanoplatform was used to specifically deliver SN38 to colorectal cancer cells. The results demonstrated that the synthesized conjugate was self-assembled to nanoparticles with 169 nm in size and poly dispersity index of 0.11. Besides, the targeted self-assembled nanoparticles could significantly enhance the cellular uptake by CD133-expressing HT29 cell line confirmed by fluorescent microscopy and flow cytometry. Moreover, our results revealed that the targeted self-assembled nanoconjugate exhibited significantly lower IC

Topics: AC133 Antigen; Animals; Biological Transport; Carboxymethylcellulose Sodium; CHO Cells; Colorectal Neoplasms; Cricetulus; Drug Carriers; Drug Liberation; HT29 Cells; Humans; Irinotecan; Micelles; Nanoparticles; Polyethylene Glycols; Solubility

Auto-crosslinked polysaccharide hyaluronan-based solution (Hyalobarrier-gel) prevents postoperative adhesions. However, its effect on tumour growth is still unknown. The aim of the present study was therefore to investigate the impact on survival of intra-abdominally administered Hyalobarrier-gel, native hyaluronan (HA) and hyaluronan/carboxymethylcellulose (HA/CMC), after intraperitoneal tumour implantation.. After receiving an intraperitoneal inoculum of the human HT29 colorectal cell line, 615 athymic nude mice were assigned randomly to five groups: groups 1 and 2 received Hyalobarrier-gel 20 mg/ml (n = 124) and 40 mg/ml (n = 126) respectively; groups 3 and 4 received HA (n = 120) and HA/CMC film (Seprafilm) (n = 123) respectively. The survival of each treated group was compared with that of group 5, the control, which had no treatment (n = 122).. As 34 of the 615 mice were not eligible, 581 animals were considered for the analysis. At 120 days, 136 animals (23.4 per cent) were still alive. At autopsy there was macroscopic absence of tumour in 75 cases (12.9 per cent). No statistically significant differences were found between the treatment and the control groups with respect to postoperative death and absence of tumour implantation. There was no difference in survival rate between the control group and groups treated with Hyalobarrier-gel, HA or HA/CMC.. Hyalobarrier-gel, HA and HA/CMC had no negative impact on the survival rate in mice that received an intraperitoneal implantation of HT29 colorectal human tumour cells.

Topics: Animals; Anticarcinogenic Agents; Carboxymethylcellulose Sodium; Colorectal Neoplasms; Drug Combinations; Gels; HT29 Cells; Humans; Hyaluronic Acid; Male; Mice; Mice, Nude; Neoplasm Transplantation; Peritoneal Neoplasms; Random Allocation; Survival Analysis; Tissue Adhesions