op-41483-alpha-cyclodextrin and Pulmonary-Edema

It is not yet known whether a prostacyclin analogue can affect alveolar fluid clearance. According to recent studies, high-dose (10(-3) M) terbutaline, a beta(2)-adrenergic agonist, failed to increase alveolar fluid clearance. Therefore, we examined the effects of OP-41483alpha-CD, a prostacyclin analogue, on alveolar fluid clearance in the presence of high-dose terbutaline in rats.. Albumin solution containing Evans blue dye and various drugs was instilled into the alveolar airspaces of isolated rat lungs, which were then inflated with 100% oxygen at an airway pressure of 8 cmH(2)O. Alveolar fluid clearance was measured by the progressive increase in dye concentrations over 1 h.. Although 10(-5) and 10(-4) M terbutaline increased alveolar fluid clearance, 10(-3) M terbutaline did not. OP-41483alpha-CD restored the ability of 10(-3) M terbutaline to stimulate alveolar fluid clearance. The effect of OP-41483alpha-CD was consistent with the effect of atenolol, a beta(1)-adrenergic antagonist. The effect of OP-41483alpha-CD on alveolar fluid clearance was unchanged in lungs inflated with nitrogen. Prostaglandin E (PGE)(1) and PGE(2) analogues had similar effects to OP-41483alpha-CD on alveolar fluid clearance.. These results indicate that a prostacyclin analogue restores the ability of high-dose terbutaline to stimulate alveolar fluid clearance.

Topics: Adrenergic beta-Agonists; Adrenergic beta-Antagonists; alpha-Cyclodextrins; Animals; Atenolol; Cyclodextrins; Epithelial Cells; Epoprostenol; Pulmonary Alveoli; Pulmonary Edema; Rats; Rats, Sprague-Dawley; Terbutaline

Topics: alpha-Cyclodextrins; Animals; Blood Gas Analysis; Cyclodextrins; Dextrins; Dogs; Epoprostenol; Hemodynamics; Hyperbaric Oxygenation; In Vitro Techniques; Perfusion; Pulmonary Edema; Starch