olivomycins and Carcinoma--Ehrlich-Tumor

Topics: Animals; Antibiotics, Antineoplastic; Carcinoma, Ehrlich Tumor; Child; Choriocarcinoma; Culture Techniques; Dactinomycin; Female; Hodgkin Disease; Humans; Leukemia, Experimental; Leukemia, Lymphoid; Lymphoma, Non-Hodgkin; Neoplasms; Olivomycins; Polycythemia; Pregnancy; Sarcoma 180; Tonsillar Neoplasms; USSR; Wilms Tumor

Efficiency and duration of suppression of the synthesis of low-polymer (4S) and ribosomal (18S and 28S) fractions of total RNA due to exposure of the cells of Ehrlich's carcinoma and Zajdela's hepatoma to olivomycin were studied. The tumors differed in their sensitivity to the antibiotic. The efficiency of the rRNA synthesis inhibition by olivomycin on its addition to the cells of Ehrlich's carcinoma and Zajdela's hepatoma in vitro was of the same order. Study on the kinetics of rRNA synthesis after addition of olivomycin showed that the maximum inhibition of rRNA synthesis in the cells of Ehrlich's carcinoma was achieved 1 hour after the drug addition. After 2 hours the inhibition level returned to normal. The most efficient inhibition of rRNA synthesis in the cells of Zajdela's hepatoma was observed 2 hours after addition of the drug. The inhibition level in this case did not return to normal after 24 hours. It is concluded that tumor sensitivity to olivomycin is determined by the duration of the affection of the DNA template activity during the synthesis of RNA.

Topics: Animals; Carcinoma, Ehrlich Tumor; Drug Resistance; Female; Kinetics; Liver Neoplasms, Experimental; Mice; Mice, Inbred Strains; Olivomycins; RNA, Neoplasm; RNA, Ribosomal; Time Factors

The pharmacokinetics of C14-olivomycin after its single intravenous administration to mice with lymphosarcome (LIO-I) was studied. It was shown that according to the specific radioactivity the organs may be placed in the following order: I hour after the antibiotic administration-the blood, liver, spleen, thymus, tumor, muscle; 3 hour after the administration-the liver, spleen, thymus, blood, tumor, muscle. Accumulation of olivomycin in the mouse organs was mainly in direct dependence on the dose of the antibiotic administered. Chromatography of the substances extraceted with ethylacetate from the urine collected at various periods after C14-olivomycin administration showed the presence of a new radioactive product (Rf 0.35-0.37) in addition to the unchanged antibiotic (Rf 0.53). Bioautographic analysis of the chromatograms showed that the product of C14-olivomycin conversion preserved its biological activity. The analysis of the substances extracted with ethylacetate from the liver, spleen and tumors 3 hours after the antibiotic administration reveiled (except of the liver) the presence of a spot with Rf corresponding to that of the initial drug.

Topics: Animals; Autoradiography; Carbon Radioisotopes; Carcinoma, Ehrlich Tumor; Chromatography, Paper; Dose-Response Relationship, Drug; Injections, Intravenous; Kinetics; Lymphoma, Non-Hodgkin; Male; Mice; Neoplasm Transplantation; Olivomycins; Radiochemistry; Sarcoma, Experimental; Scintillation Counting; Spectrophotometry; Time Factors

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antineoplastic Agents; Busulfan; Carcinoma, Ehrlich Tumor; Leukemia; Leukemia, Myeloid; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms, Experimental; Nitrogen Mustard Compounds; Olivomycins; Pharmacology; Research; Sarcoma 180; Thiotepa; Toxicology

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antitubercular; Carcinoma; Carcinoma, Ehrlich Tumor; Dermatologic Agents; Olivomycins