oligomycins and Anaphylaxis

Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization of ATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 pmol/10(3) cells. 2.5 min after cell activation, the rate of additional ATP-utilization was 0.30 pmol/10(3) cells/min, and the persistent ATP-decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP-synthesis.

Topics: Adenosine Triphosphate; Anaphylaxis; Animals; Antimycin A; Deoxyglucose; Energy Metabolism; Exocytosis; Histamine Release; In Vitro Techniques; Male; Mast Cells; Oligomycins; Rats; Rats, Inbred Strains

Topics: Adenosine Triphosphate; Anaphylaxis; Animals; Antigen-Antibody Reactions; Deoxyglucose; Ethanol; Histamine Release; In Vitro Techniques; Mast Cells; Oligomycins; Rats

The ATP content of rat peritoneal mast cells has been studied in relation to histamine release induced by compound 48/80 and antigen-antibody (anaphylactic) reaction in vitro. When the ATP content of actively sensitized mast cells was reduced to different levels by oligomycin, a good correlation was obtained between the ATP levels and the amounts of histamine released by the anaphylactic reaction. A similar linear relation has previously been demonstrated between the ATP levels of mast cells and histamine release induced by compound 48/80. The ATP content of mast cells was also studied at different intervals after the exposure of the cells to antigen or compound 48/80. No significant change in the ATP content was observed in untreated mast cells during the short period when histamine release occurs. If, however, the mast cells were preincubated with oligomycin or 2-deoxyglucose to reduce the rate of ATP synthesis while a large part of the histamine release remained unaffected-a decrease in the ATP content could be demonstrated in close time relation to both anaphylactic and compound 48/80-induced histamine release. The observations indicate an increased utilization of ATP in mast cells during the release process.

Topics: Adenosine Triphosphate; Anaphylaxis; Animals; Antigen-Antibody Reactions; Antigens; Deoxyglucose; Histamine Release; Male; Mast Cells; Oligomycins; p-Methoxy-N-methylphenethylamine; Rats; Time Factors

Topics: Aerobiosis; Anaerobiosis; Anaphylaxis; Animals; Carbon Dioxide; Carbon Radioisotopes; Dextrans; Glucose; Helium; Histamine Release; Lactates; Mast Cells; Nitrogen; Oligomycins; Ovalbumin; Oxidation-Reduction; Oxygen Consumption; p-Methoxy-N-methylphenethylamine; Phosphatidylserines; Rats