oligomycins and Alcoholism

The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding proton-motive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative-phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol. Cytochrome oxidase inhibition by sodium-azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation.

Topics: Alcohol Drinking; Alcoholic Intoxication; Alcoholism; Animals; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Electron Transport Complex IV; Hydrogen-Ion Concentration; Kinetics; Male; Mitochondria, Liver; Oligomycins; Oxidative Phosphorylation; Oxygen Consumption; Rats; Rats, Wistar; Rotenone; Sodium Azide

Topics: Alcoholism; Animals; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Ethanol; Humans; Male; Mitochondria; Mitochondria, Liver; Oligomycins; Oxidative Phosphorylation; Oxygen Consumption; Rats; Rotenone; Submitochondrial Particles