oleuropein and Pleurisy

Several olive oil phenolic compounds, such us oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this study was to investigate the effects of oleuropein aglycone, a hydrolysis product of oleuropein, in a mouse model of carrageenan-induced pleurisy.. Mice were anaesthetized and subjected to a skin incision at the level of the left sixth intercostals space. The underlying muscle was dissected and saline or saline containing 2% λ-carrageenan was injected into the pleural cavity.. Injection of carrageenan elicited an acute inflammatory response characterized by: infiltration of neutrophils in lung tissues (P < 0.01 versus sham. P < 0.01 versus carrageenan) and subsequent lipid peroxidation (P < 0.01 versus sham. P < 0.01 versus carrageenan), increased production of tumor necrosis factor-α and interleukin-1β (P < 0.01 versus sham. P < 0.01 versus carrageenan), increased expression of adhesion molecules, increased synthesis of nitric oxide (P < 0.01 versus sham. P < 0.01 versus carrageenan), nitrotyrosine and poly-ADP-ribose (P < 0.01 versus sham. P < 0.01 versus carrageenan). Administration of oleuropein aglycone 30 min after the challenge with carrageenan, caused a significant reduction of all the parameters of inflammation measured.. Thus, we propose that olive oil phenolic constituents may be useful in the treatment of various inflammatory diseases.

Topics: Animals; Anti-Inflammatory Agents; Antioxidants; Carrageenan; Disease Models, Animal; Hydrolysis; Intercellular Adhesion Molecule-1; Interleukin-1beta; Iridoid Glucosides; Iridoids; Lipid Peroxidation; Lung; Male; Mice; Nitric Oxide; Olive Oil; P-Selectin; Phenols; Plant Oils; Pleurisy; Poly Adenosine Diphosphate Ribose; Pyrans; Tumor Necrosis Factor-alpha; Tyrosine