oleuropein and Osteosarcoma

Topics: Anti-Infective Agents; Antibiotics, Antineoplastic; Cell Proliferation; Cell Survival; Cytotoxins; Dietary Supplements; Doxorubicin; Humans; Iridoid Glucosides; Iridoids; Osteosarcoma; Tumor Cells, Cultured

Oleuropein belongs to the potent polyphenols of olive oil. Notably, it is considered as a potentially active anticancer agent. Herein, the anticancer efficiency of oleuropein, when used separately and in combination with the chemotherapeutic agent, 2-methoxyestradiol (2-ME), was investigated in highly metastatic osteosarcoma (OS) cells.. Human OS cells (143B OS cell line) were incubated with oleuropein and 2-ME, alone or in combination. Cell viability was determined by the MTT assay. Cell migration assays were used in order to determine the anti-migratory potential of the compounds, while their impact on autophagy was evaluated via the LC3-antibody-based detection assay. The interaction between oleuropein and 2-ME was determined via the CalcuSyn software.. Both anti-migratory and anti-proliferative effects of oleuropein were demonstrated on human OS cells. Anticancer effects of oleuropein were significantly enhanced after 2-ME addition. Treatment of 143B OS cell with oleuropein, alone or in combination with 2-ME resulted in induction of autophagy.. The obtained data suggest an anticancer effect of oleuropein, alone and in combination with 2-ME, on highly metastatic 143B OS cells. Notably, a synergism between oleuropein and 2-ME towards 143B OS cells was detected. The exact mechanism of this synergism needs to be further investigated; nonetheless, induction of nitro-oxidative stress and/or induction of autophagy are suggested.

Topics: 2-Methoxyestradiol; Antineoplastic Agents; Bone Neoplasms; Cell Line, Tumor; Cell Movement; Cell Survival; Drug Synergism; Humans; Iridoid Glucosides; Iridoids; Olive Oil; Osteosarcoma

In this study, we evaluated the antiproliferative activity on two human osteosarcoma cell lines (MG-63 and Saos2) of oleuropein, an olive oil compound traditionally found in the Mediterranean diet. Oleuropein exhibited obvious cytotoxic effects on human osteosarcoma cells in a concentration- and time-dependent manner. Statistical analysis of IC50 by the Probit regression method suggested that oleuropein had similar toxic effects on both cell lines tested (IC50 range from 247.4-475.0 µM for MG63 cells and from 798.7-359.9 µM for Saos2 cells).

Topics: Cell Line, Tumor; Cell Proliferation; Humans; Iridoid Glucosides; Iridoids; Osteosarcoma; Phytotherapy; Plant Extracts