oleanane and Leukemia--Basophilic--Acute

The methanolic extract and its 1-butanol-soluble fraction from the flower buds of Camellia sasanqua THUNB. were found to show inhibitory activities on the release of β-hexosaminidase from rat basophile leukemia (RBL-2H3) cells. From the 1-butanol-soluble fraction, five new acylated oleanane-type triterpene saponins, sasanquasaponins I-V, were isolated together with a known saponin and their chemical structures were elucidated on the basis of chemical and physicochemical evidence. The principal saponin constituents, sasanquasaponins I-III, with an acyl group at the 22-position of the aglycon part showed the inhibitory effects on the release of β-hexosaminidase and some structure-activity relationships were reported.

Topics: Animals; Anti-Allergic Agents; beta-N-Acetylhexosaminidases; Camellia; Cell Line, Tumor; Flowers; Leukemia, Basophilic, Acute; Magnetic Resonance Spectroscopy; Molecular Conformation; Oleanolic Acid; Rats; Saponins; Structure-Activity Relationship; Triterpenes