okadaic-acid has been researched along with Laryngeal-Neoplasms* in 1 studies
1 other study(ies) available for okadaic-acid and Laryngeal-Neoplasms
| Article | Year |
|---|---|
Evaluation of the use of two human cell lines for okadaic acid and DTX-1 determination by cytotoxicity assays and damage characterization.
Two human cell lines have been used, HEp-2 and (de)differentiated Caco-2, derived from a larynx and a colon carcinoma, respectively, with the aim of evaluating and characterizing the cytotoxicity of okadaic acid (OA) and related toxins. Effects of OA and dinophysistoxin-1 (DTX-1) on cell viability (neutral red uptake) and on cell morphology/cytoskeleton structure have been observed in both cell lines, though at different time exposures and with different concentrations. The morphological alteration was detected earlier than the viability inhibition in HEp-2 cells with both toxins and in Caco-2 cells with DTX-1. HEp-2 cells have shown to be more sensitive than the intestinal cell line and thus possibly suitable for screening of contaminated samples, while Caco-2 cells could be used for further investigating the possible mechanisms involved in diarrhoeic shellfish poisoning (DSP) toxins. Topics: Apoptosis; Caco-2 Cells; Cell Nucleus; Cell Survival; Coloring Agents; Cytoskeleton; Epithelial Cells; Fluorescent Antibody Technique; Humans; Immunohistochemistry; Inhibitory Concentration 50; Laryngeal Neoplasms; Marine Toxins; Neutral Red; Okadaic Acid; Pyrans; Tubulin; Tumor Cells, Cultured | 1998 |