obelin and Tuberculosis

obelin has been researched along with Tuberculosis* in 1 studies

Other Studies

1 other study(ies) available for obelin and Tuberculosis

Article	Year
Use of proZZ-obelin fusion protein in bioluminescent immunoassay. Biochemical and biophysical research communications, 1996, Feb-15, Volume: 219, Issue:2 Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coli by recombinant DNA techniques. The proZZ-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 X 10(15) photons per mg of protein. Topics: Animals; Antibodies, Bacterial; Calcium; Escherichia coli; Humans; Immunoglobulin G; Immunoglobulins; Kinetics; Luminescent Measurements; Luminescent Proteins; Mice; Plasmids; Rabbits; Recombinant Fusion Proteins; Restriction Mapping; Tuberculosis	1996

Article

Year

Use of proZZ-obelin fusion protein in bioluminescent immunoassay.

Biochemical and biophysical research communications, 1996, Feb-15, Volume: 219, Issue:2

Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coli by recombinant DNA techniques. The proZZ-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 X 10(15) photons per mg of protein.

Topics: Animals; Antibodies, Bacterial; Calcium; Escherichia coli; Humans; Immunoglobulin G; Immunoglobulins; Kinetics; Luminescent Measurements; Luminescent Proteins; Mice; Plasmids; Rabbits; Recombinant Fusion Proteins; Restriction Mapping; Tuberculosis

1996