nystatin-a1 and Escherichia-coli-Infections

Extra-intestinal pathogenic Escherichia coli (ExPEC) strains are responsible for a wide range of diseases in humans and animals. Using in vitro invasion assays and transmission electron microscopy, we showed that BEN2908, an ExPEC strain of avian origin (also termed APEC for Avian Pathogenic E. coli), is able to usurp cellular endocytic pathways to invade A549 human type II pneumocytes and LMH avian hepatocytes where it is able to survive over several days. Although type 1 fimbriae are the major adhesin of BEN2908, proportions of adherent fimbriated or afimbriated bacteria that entered cells were comparable. Internalization of BEN2908 into human pneumocytes reinforces previous studies indicating that APEC strains could represent a zoonotic risk.

Topics: Alveolar Epithelial Cells; Animals; Anti-Bacterial Agents; Birds; Cell Line; Chlorpromazine; Cytochalasin D; Endocytosis; Epithelial Cells; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Filipin; Fimbriae, Bacterial; Hepatocytes; Humans; Microbial Viability; Microscopy, Electron, Transmission; Nystatin; Time Factors

Trimethoprim-sulfamethoxazole (TMP-SMX) is a combination chemotherapeutic agent, a commonly used antibiotic. Adverse drug reactions occur in 6-8% of patients. Although, the most common adverse reactions include mild gastrointestinal distress and cutaneous events, also a wide range of hematological abnormalities have been ascribed to TMP-SMX. We report a 40-year-old male patient who developed an early onset neutropenia, thrombocytopenia, generalised rash and oral candidiasis after 5 days long TMP-SMX therapy. Although generalised rash may seen more and improves with discontinuation of the therapy; severe neutropenia, thrombocytopenia and oral candidiasis are seen very rare and rarely leads to fatality as it was in our case. Despite thrombocyte transfusions, whole blood transfusions, red cell concentrates and filgrastim therapy we lost our patient. We want to underline that although the TMP-SMX combination is usually well tolerated it can also lead to fatal complications.

Topics: Adult; Anti-Infective Agents; Anti-Inflammatory Agents; Candidiasis, Oral; Cefepime; Cephalosporins; Chlorhexidine; Drug Eruptions; Escherichia coli; Escherichia coli Infections; Exanthema; Fatal Outcome; Filgrastim; Granulocyte Colony-Stimulating Factor; Humans; Male; Meropenem; Neutropenia; Nystatin; Platelet Transfusion; Prednisolone; Recombinant Proteins; Thienamycins; Thrombocytopenia; Trimethoprim, Sulfamethoxazole Drug Combination

Topics: Amphotericin B; Animals; Anti-Bacterial Agents; Anti-Infective Agents; Bacterial Infections; Candida albicans; Candidiasis; Escherichia coli; Escherichia coli Infections; Mice; Neomycin; Nystatin; Penicillins; Polymyxins; Staphylococcal Infections; Streptomycin; Tetracycline; Thiourea; Undecylenic Acids

Topics: Adult; Ampicillin; Anti-Bacterial Agents; Antifungal Agents; Appendicitis; Escherichia coli Infections; Humans; Kanamycin; Male; Methicillin; Nystatin; Peritonitis; Polymyxins; Staphylococcal Infections; Sulfadimethoxine; Tetracycline