nystatin-a1 has been researched along with Enterovirus-Infections* in 1 studies
1 other study(ies) available for nystatin-a1 and Enterovirus-Infections
Article | Year |
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A novel cell entry pathway for a DAF-using human enterovirus is dependent on lipid rafts.
The glycosylphosphatidylinositol (GPI)-anchored complement regulatory protein decay-accelerating factor (DAF) is used by a number of enteroviruses as a receptor during infection. DAF and other GPI-anchored proteins can be found in cholesterol-rich ordered domains within the plasma membrane that are known as "lipid rafts." We have shown, by using drugs to specifically inhibit various endocytosis routes, that infection by a DAF-using strain of echovirus 11 (EV11) is dependent upon cholesterol and an intact cytoskeleton, whereas a non-DAF-using mutant derived from it was unaffected by these drugs. Using RNA transfection and virus-binding assays, we have shown that this requirement for cholesterol, the actin cytoskeleton, and the microtubule network occurs postbinding of the virus but prior to uncoating of the RNA, indicating a role during virus entry. Confocal microscopy of virus infection supported the role of cholesterol and the cytoskeleton during entry. In addition, [(35)S]methionine-labeled DAF-using EV11, but not the non-DAF-using EV11, could be copurified with lipid raft components during infection after Triton X-100 extraction. These data indicate that DAF usage by EV11 enables the virus to associate with lipid rafts and enter cells through this novel route. Topics: Caveolin 1; Caveolins; CD55 Antigens; Cell Line; Chlorpromazine; Cholesterol; Cytochalasin D; Cytoskeleton; Endocytosis; Enterovirus B, Human; Enterovirus Infections; Humans; Membrane Microdomains; Nocodazole; Nystatin | 2002 |