nystatin-a1 and Carcinoma--Ehrlich-Tumor

Treatment of transformed Py3T3, SV101-3T3, and L1210 cells, as well as mitotic and Pronase-treated untransformed 3T3 cells, with the polyene antibiotics filipin, nystatin, and amphotericin B inhibited agglutination by wheat germ agglutinin. The effect of polyene antibiotic treatment was lectin and cell specific. Concanavalin A induced agglutination was not inhibited, wheat germ agglutination induced agglutination of untransformed 3T3 interphase cells was not influenced, and other aggregation phenomena, including those of erythrocytes with blood group specific antibodies or divalent cations, were unaffected by polyene treatments. This suggests that the formation of polyene-cholesterol complexes in transformed and erythrocyte cell membranes may specifically affect wheat germ agglutinin receptors and/or secondary events necessary for wheat germ agglutinin induced agglutination. Fluorescence studies of membrane filipin-cholesterol complexes showed that pretreating the cells with wheat germ agglutinin, but not concanavalin A, perturbed the fluorescence properties of filipin. Electron spin resonance studies with spin-labeled fatty acids revealed at best only a slight decrease in fatty acyl chain flexibility following filipin treatment. These studies indicate that there are not only quantitative differences between the agglutinability of transformed and untransformed cells with wheat germ agglutinin but that qualitative differences exist as well.

Topics: Agglutination; Amphotericin B; Animals; Carcinoma, Ehrlich Tumor; Cell Transformation, Viral; Cells, Cultured; Concanavalin A; Fibroblasts; Filipin; Lectins; Leukemia L1210; Mice; Mice, Inbred Strains; Mitosis; Nystatin; Polyenes; Structure-Activity Relationship

The experiments in vitro have demonstrated that an application of 0.1 mg/ml of nistatine into ascitic cells suspension would result in swelling of cells and 80% loss of intracellular potassium, associated with a considerable reduction of Na+K+-ATP-ase activity, the intensity of respiration and glycosis being changed but insignificantly. Thus, the influence of a polyene antibiotic - nistatine on the water-salt balance of ascitic cells under study is likely to be somewhat related with inhibition of NA+K+-dependent ATP-ase.

Topics: Adenosine Triphosphatases; Animals; Antibiotics, Antineoplastic; Ascitic Fluid; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; Female; Glycolysis; In Vitro Techniques; Liver Neoplasms; Mice; Neoplasms, Experimental; Nystatin; Ovarian Neoplasms; Oxygen Consumption; Potassium; Sodium