nu-7026 and Liver-Neoplasms

nu-7026 has been researched along with Liver-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for nu-7026 and Liver-Neoplasms

Article	Year
The effect of inhibitors of phosphatidylinositol 3-kinase-related kinases on dibenzo[def,p]chrysene genotoxicity measured by γH2AX levels and neutral comet assay in HepG2 human hepatocellular cancer cells. Toxicology in vitro : an international journal published in association with BIBRA, 2020, Volume: 63 In the study the modulating effect of inhibition of phosphatidylinositol 3-kinase-related kinases (PIKK): ATM (Ataxia Telangiectasia Mutated), ATR (Ataxia Telangiectasia and Rad3 Related) and DNA-PK (DNA-dependent protein kinase) on genotoxicity of dibenzo[def,p]chrysene (DBC) in HepG2 human hepatocellular cancer cells was investigated. The cytotoxicity of DBC was determined, also in combination with PIKK inhibitors, using the MTT reduction assay. The high cytotoxicity of DBC was observed after 72 h incubation (IC Topics: Benzopyrenes; Carcinoma, Hepatocellular; Cell Survival; Chromones; Comet Assay; DNA Damage; Hep G2 Cells; Histones; Humans; Liver Neoplasms; Morpholines; Phosphatidylinositol 3-Kinase; Protein Kinase Inhibitors	2020
The modulating effect of ATM, ATR, DNA-PK inhibitors on the cytotoxicity and genotoxicity of benzo[a]pyrene in human hepatocellular cancer cell line HepG2. Environmental toxicology and pharmacology, 2015, Volume: 40, Issue:3 The effect of inhibitors of phosphatidylinositol-3-kinase-related kinases (PIKK): ataxia-telangiectasia mutated (ATM), ATM- and Rad3-related (ATR) and DNA-dependent protein kinase (DNA-PK) on response of HepG2 human liver cancer cells to benzo[a]pyrene (BaP) was investigated. PIKK inhibitors: KU55933 (5 μM), NU7026 (10 μM) or caffeine (1 and 2mM) when used as single agents or in combinations (KU55933/NU7026 and caffeine/NU7026) did not significantly influence the BaP (3 μM) cytotoxicity (MTT reduction test). BaP induced a weak proapoptotic effect which was moderately enhanced by both inhibitor combinations. HepG2 cells exposed to BaP showed a strong S-phase arrest which was considerably diminished by both inhibitor combinations. The DNA damage (comet assay) induced after continuous 24h exposure to BaP was significantly diminished by both inhibitor combinations. Weak induction of reactive oxygen species by BaP was observed, which was not modulated by the inhibitor combinations. Similarly, no modulation of the glutathione levels was observed. Topics: Apoptosis; Ataxia Telangiectasia Mutated Proteins; Benzo(a)pyrene; Caffeine; Cell Survival; Chromones; DNA Damage; Hep G2 Cells; Humans; Liver Neoplasms; Morpholines; Protein Kinase Inhibitors; Protein Serine-Threonine Kinases; Pyrones	2015

Article

Year

The effect of inhibitors of phosphatidylinositol 3-kinase-related kinases on dibenzo[def,p]chrysene genotoxicity measured by γH2AX levels and neutral comet assay in HepG2 human hepatocellular cancer cells.

Toxicology in vitro : an international journal published in association with BIBRA, 2020, Volume: 63

In the study the modulating effect of inhibition of phosphatidylinositol 3-kinase-related kinases (PIKK): ATM (Ataxia Telangiectasia Mutated), ATR (Ataxia Telangiectasia and Rad3 Related) and DNA-PK (DNA-dependent protein kinase) on genotoxicity of dibenzo[def,p]chrysene (DBC) in HepG2 human hepatocellular cancer cells was investigated. The cytotoxicity of DBC was determined, also in combination with PIKK inhibitors, using the MTT reduction assay. The high cytotoxicity of DBC was observed after 72 h incubation (IC

Topics: Benzopyrenes; Carcinoma, Hepatocellular; Cell Survival; Chromones; Comet Assay; DNA Damage; Hep G2 Cells; Histones; Humans; Liver Neoplasms; Morpholines; Phosphatidylinositol 3-Kinase; Protein Kinase Inhibitors

2020

The modulating effect of ATM, ATR, DNA-PK inhibitors on the cytotoxicity and genotoxicity of benzo[a]pyrene in human hepatocellular cancer cell line HepG2.

Environmental toxicology and pharmacology, 2015, Volume: 40, Issue:3

The effect of inhibitors of phosphatidylinositol-3-kinase-related kinases (PIKK): ataxia-telangiectasia mutated (ATM), ATM- and Rad3-related (ATR) and DNA-dependent protein kinase (DNA-PK) on response of HepG2 human liver cancer cells to benzo[a]pyrene (BaP) was investigated. PIKK inhibitors: KU55933 (5 μM), NU7026 (10 μM) or caffeine (1 and 2mM) when used as single agents or in combinations (KU55933/NU7026 and caffeine/NU7026) did not significantly influence the BaP (3 μM) cytotoxicity (MTT reduction test). BaP induced a weak proapoptotic effect which was moderately enhanced by both inhibitor combinations. HepG2 cells exposed to BaP showed a strong S-phase arrest which was considerably diminished by both inhibitor combinations. The DNA damage (comet assay) induced after continuous 24h exposure to BaP was significantly diminished by both inhibitor combinations. Weak induction of reactive oxygen species by BaP was observed, which was not modulated by the inhibitor combinations. Similarly, no modulation of the glutathione levels was observed.

Topics: Apoptosis; Ataxia Telangiectasia Mutated Proteins; Benzo(a)pyrene; Caffeine; Cell Survival; Chromones; DNA Damage; Hep G2 Cells; Humans; Liver Neoplasms; Morpholines; Protein Kinase Inhibitors; Protein Serine-Threonine Kinases; Pyrones

2015