nsc-4347 and Diarrhea

Post-weaning bacterial infections in piglets caused by resistant strains have increased dramatically. Thai medicinal plants such as Piper retrofractum, Piper leptostachyum, Piper sarmentosum, Zingiber officinale, Plumbago indica, Piper betle, Caesalpinia sappan, Garcinia mangostana etc have been widely used in Thai traditional medicine to treat diarrhea. Thus, these plants were investigated antimicrobial activity against gram negative strain isolated from piglets with diarrhea.. To investigate antimicrobial activity of Thai medicinal plants and their isolated compounds in order to develop an alternative treatment against bacteria causing post-weaning diarrhea in piglets.. Antimicrobial activity of some Thai medicinal plants and their compounds were tested using disc\ diffusion and broth dilution methods against bacteria associated with diarrheal disease including Escherichia coli and Salmonella spp. The extraction was performed by maceration in 95% ethanol.. The results showed that all tested strains were sensitive to P. betle extract. As well as Plumbagin compound from Plumbago indica also showed antimicrobial activity against all microbes the same of P. betle extract with MIC between 5 to 10 mg/ml, which are different from the group of antibiotics with MIC values between 0.19-2.5 mg/ml, P. betle extracts inhibited Escherichia coli and Salmonella spp while some antibiotics can inhibit only some types.. The results support the use of Thai medicinal plants for treatment of diarrhea caused by these bacteria. This study also provides basic knowledge on antimicrobial activity against diarrheal microbe isolated from piglets. These results lead to further development of an effective formula of Thai medicinal plants for diarrheal disease in post-weaned piglets and other infectious diseases in the future.

Topics: Anti-Bacterial Agents; Bacteria; Diarrhea; Escherichia coli; Garcinia mangostana; Humans; Medicine, Traditional; Piper; Plant Extracts; Plants, Medicinal; Plumbaginaceae; Thailand; Zingiber officinale

Bacterial infections caused by resistant strains have been increased dramatically. Pikutbenjakul, a Thai medicinal plant formula containing Piper longum, Piper sarmentosum, Piper interruptum, Plumbago indica and Zingiber officinale have been widely used in Thai traditional medicine.. To determine antimicrobial activity of Pikutbenjakul formula and its components in order to develop the medicinal plants for alternative treatment of bacteria causing diarrhea.. Activity of Pikutbenjakul formula and its components was tested using disc diffusion and broth dilution methods against bacteria associated a set of bacteria associated with diarrheal disease including Vibrio cholerae, Vibrio vulnificus, Salmonella, Shigella, Escherichia coli (EIEC, ETEC, EPEC, EAEC and EHEC) and Staphylococcus aureus. The extraction was performed by maceration in 95% ethanol.. The results showed all tested strains were susceptible to P. indica while other components were able to inhibit some strains. P. sarmentosum showed antimicrobial activity against Vibrios with the MIC values between 0.625 to > or =5 mg/ml. P. sarmentosum, P. indica and Pikutbenjakul formulas inhibited the growth of all Vibrios. P. interruptum inhibited V. cholerae serogroups O1 and non-O1/non-O139. P. longum was able to inhibit only two isolates of V. cholerae serogroup O139 (MIC = 1.25 mg/ml) and V. vulnificus (MIC > or =5 mg/ml). The activity of Pikutbenjakul containing Zingiber spp. and Pikutbenjakul containing Z. officinal against Vibrios, Shigella spp. and S. aureus was not significantly different. P. indica could inhibit Salmonella (MIC > or =5 mg/ml), E. coli (MIC > or =5 mg/ml) and S. aureus (MIC = 1.25 mg/ml).. The results support the Thai medicinal plants for treatment of diarrhea caused by these bacteria. This study also provides an insightful knowledge on antimicrobial activity which would lead to further development of an effective formula of Pikutbenjakul for diarrheal disease and other infectious diseases in future.

Topics: Anti-Bacterial Agents; Diarrhea; Disk Diffusion Antimicrobial Tests; Ethanol; Gram-Negative Bacteria; Hospitals, University; Humans; Medicine, Traditional; Phytotherapy; Piper; Plant Extracts; Plants, Medicinal; Plumbaginaceae; Staphylococcus aureus; Thailand; Zingiber officinale