nsc-141537 and Body-Weight

A hydrated sodium calcium aluminosilicate (HSCAS) was incorporated into diets (.5%) containing 3.5 mg/kg aflatoxin (AF) and 5.0 mg/kg diacetoxyscirpenol (DAS) singly and in combination. Male broiler chicks received ad libitum access to their respective diets and water from 1 to 21 days of age. Body weight gains were significantly (P < .05) depressed by AF and DAS singly and a synergistic interaction occurred between AF and DAS for a further depression of body weight gains. Alterations in hematological and serum biochemical values, as well as serum enzyme activities, were observed for the AF and the AF and DAS combination. Additionally, a significant interaction occurred between AF and DAS for some biochemical values and enzyme activities. Adding HSCAS resulted in almost total protection against the effects caused by AF alone, limited protection against the combination, but no protection against the DAS alone. These findings indicate that HSCAS can diminish the adverse effects of AF but not of DAS.

Topics: Aflatoxins; Aluminum Silicates; Analysis of Variance; Animal Feed; Animals; Body Weight; Chickens; Male; Organ Size; Trichothecenes; Zeolites

Three experiments were conducted to determine the effects of 4,15-diacetoxyscirpenol (DAS) on BW, feed consumption, and oral lesions of broiler breeders. In Experiment 1, caged broiler breeder hens were fed 0, 5, 10, or 20 mg DAS/kg diet from 24 to 25 wk of age. There were dose-related decreases in BW and feed consumption indicating feed refusal, as well as dose-related increases in the extent of mouth lesions. The areas of the mouth most sensitive to DAS were associated with the salivary glands and the tip of the tongue. In Experiment 2, individually caged male and female broiler breeders were fed a basal diet containing 0, 5, 10, or 20 mg DAS/kg from 25 to 27 wk of age. There were dose-related decreases in BW and feed consumption for the female broiler breeders, whereas there was a decrease in feed consumption for the male broiler breeders at the 10 and 20 mg DAS/kg levels. In Experiment 3, male broiler breeders were fed 0 or 10 mg DAS/kg diet from 23 to 25 wk of age on a litter floor. For this experiment the daily intake of feed was restricted, and the feed consumption rate was measured. There was an increased amount of unconsumed feed at 23 wk of age due to the presence of DAS. In summary, the experiments provided evidence that DAS caused decreased BW and feed consumption as well as cytotoxic injury including oral lesions in broiler breeders.

Topics: Animals; Body Weight; Chickens; Eating; Female; Male; Mouth Diseases; Mycotoxins; Trichothecenes

The individual and combined effects of feeding diets containing 300 mg fumonisin B1 (FB1), and 4 mg diacetoxyscirpenol (DAS) or 3 mg ochratoxin A (OA) were evaluated in two experiments using female turkey poults (Nicholas Large Whites) from day of hatch to 3 wk of age. When compared with controls, body weight gains were reduced 30% (Study 1) and 24% (Study 2) by FB1, 30% by DAS, 8% by OA, 46% by the FB1 and DAS combination, and 37% by the FB1 and OA combination. The efficiency of feed utilization was adversely affected by all treatments except FB1 in Experiment 2. Relative weights of the liver were significantly increased by all treatments except the DAS treatment. Serum concentrations of cholesterol were decreased and activities of aspartate aminotransferase and lactate dehydrogenase were increased and several hematological values were altered in poults fed FB1 alone and in combination with either DAS or OA. Results indicate additive or less than additive toxicity, but not toxic synergy, when poults are fed diets containing 300 mg FB1, and 4 mg DAS or 3 mg OA/kg of diet. The likelihood of encountering FB1, DAS, or OA at these concentrations in finished feed is small. However, under field conditions, other stress factors could alter the impact of these mycotoxins on the health and performance of poultry.

Topics: Animal Feed; Animals; Aspartate Aminotransferases; Body Weight; Carboxylic Acids; Cholesterol; Drug Interactions; Energy Metabolism; Erythrocyte Count; Female; Fumonisins; Fusarium; Hematocrit; L-Lactate Dehydrogenase; Liver; Mycotoxins; Ochratoxins; Organ Size; Trichothecenes; Triglycerides; Turkeys; Weight Gain

Topics: Aflatoxins; Analysis of Variance; Animal Feed; Animals; Blood Proteins; Blood Urea Nitrogen; Body Weight; Cholesterol; Eating; Enzymes; Female; Food Contamination; Iron; Liver; Mycotoxins; Random Allocation; Sheep; Trichothecenes

1. The individual and combined effects of T-2 toxin and 4,15-diacetoxyscirpenol (DAS) on laying hens were investigated in an experiment consisting of a 2 x 2 completely randomised factorial design with dietary concentrations of 0 and 2 mg/kg T-2 toxin and 0 and 2 mg/kg DAS. 2. Individually, T-2 toxin and DAS induced oral lesions in half of the hens and decreased significantly egg production and food intake. 3. The effects of T-2 toxin and DAS were additive for reduced food consumption and incidence of oral lesions. However, a synergism for reduced egg production was observed during the last experimental period. 4. No effects on body weight were observed during this study. Mild changes in selected plasma enzymes activities and no change in liver malondialdehyde content were detected. 5. The combination of T-2 toxin and DAS was more toxic than the single mycotoxins, for some parameters, and therefore, may pose a greater economic threat to the poultry industry than either of the toxins individually.

Topics: Animals; Body Weight; Chickens; Diet; Drug Synergism; Feeding Behavior; Female; Liver; Malondialdehyde; Mycotoxins; Organization and Administration; Oviposition; Random Allocation; T-2 Toxin; Time Factors; Trichothecenes

The effects of feeding 2 mg ochratoxin A (OA) and 6 mg 4,15-diacetoxyscirpenol (DAS)/kg of diet singly and in combination were characterized in male broiler chicks from 1 to 19 d of age. Body weights were depressed by OA, DAS, and the OA-DAS combination. There was a significant antagonistic interaction between OA and DAS for uric acid and cholesterol. The efficiency of feed utilization was reduced by DAS alone and by the OA-DAS combination. When compared with controls, additive toxicity was exhibited for reduced efficiency of feed utilization, increased relative weights of the liver and gizzard, and decreased concentration of serum total protein, mean corpuscular volume, and mean corpuscular hemoglobin. All chicks were scored for oral lesions using a scale of 1 to 4 (0 = no visible lesions; 3 = severe lesions). Oral lesions (average score = 2.6) were present in over 90% of the chicks receiving the DAS diet with or without OA. These data demonstrate that both OA and DAS alone and the OA-DAS combination can adversely affect broiler performance and health.

Topics: Animal Feed; Animals; Body Weight; Chickens; Drug Interactions; Eating; Male; Ochratoxins; Organ Size; Trichothecenes

The influence of high dietary fat on the toxicity of diacetoxyscirpenol (DAS) was investigated in a 2 x 5 factorial arrangement of treatments (6 and 12% fat, and 0, 1, 2, 4, and 8 micrograms DAS/g diet). The 3-wk body weight was decreased (P less than .0001) by DAS, but fat had no significant (P less than .05) effect. There was a highly significant (P less than .0059) interaction manifested at the higher levels of DAS by a greater decrease in body weight in the high-fat diet than in the low-fat diet. Neither feed conversion nor percentage of fat in fecal material were affected significantly (P less than .05) by DAS. These data were consistent with the high-fat diet promoting lipid micellar absorption of DAS and with DAS, once absorbed, inhibiting protein synthesis at the ribosomal level, a well established mechanism of action for trichothecene toxins such as DAS.

Topics: Animals; Body Weight; Chickens; Dietary Fats; Eating; Male; Mycotoxicosis; Mycotoxins; Poultry Diseases; Trichothecenes

Anguidine (diacetoxyscirpenol, DAS) and other trichothecene mycotoxins are potent inhibitors of protein synthesis and injure organs with rapidly dividing cell populations, including the testis. Testicular structure and function were studied in male Lewis rats 1, 3, 7, 30, 60, and 90 days after exposure at age 12 weeks to anguidine at 1.7 mg/kg body weight given by ip injection. The dose was equivalent to 75% of the ip LD50. Anguidine caused a gradual decline in testicular weight beginning 30 days after treatment. Sperm production was also reduced by 30 days, and the frequency of hypocellular seminiferous tubules increased by day 60. There was no evidence of recovery by 90 days. These changes are consistent with injury to proliferating cells early in the maturation sequence. Epididymal sperm reserves were reduced by 3 days after anguidine administration, prior to the reduction in sperm production, suggesting premature release of spermatozoa from the epididymis.

Topics: Animals; Antineoplastic Agents; Body Weight; Epididymis; Male; Organ Size; Rats; Seminiferous Tubules; Spermatids; Spermatozoa; Testicular Diseases; Testis; Trichothecenes

A characteristic of the trichothecene mycotoxin, anguidine, is its extreme toxicity to organs with populations of rapidly dividing cells. In preparation for evaluation of compounds that may protect against anguidine toxicity, we measured the LD50 of anguidine administered by gastric gavage (ig) or intraperitoneal injection (ip) and studied the dose- and time-dependent effects of anguidine on lymphohematopoietic organs, intestine, and testis, and measured hematocrit and peripheral blood leukocyte counts in male CD-1 mice. The ig LD50 at 96 hr was 15.5 mg/kg; after ip administration the LD50 at 96 hr was 20.0 mg/kg. Characteristic changes caused by sublethal doses of anguidine were cell depletion and necrosis in lymphohematopoietic organs, multifocal necrosis of intestinal epithelium, and diffuse necrosis of germinal epithelium followed by progressive tubule degeneration in the testes. There was leukocytosis due to both lymphocytosis and neutrophilia in the first few hours following anguidine exposure, followed by lymphopenia, neutropenia, and anemia by 3 days. After lethal doses, the intestinal necrosis was transmural, and there was extensive necrosis of lymphohematopoietic organs. There was rapid recovery after sublethal anguidine exposure of all anguidine-sensitive organs except for testis where decreased weights and abnormal spermatogenesis persisted for the 2-week observation period. Our results suggest that intestinal necrosis is an important cause of death following anguidine exposure. Atrophy of seminiferous tubules may have some value as an indicator of prior anguidine exposure, but the testicular changes are not unique to this compound.

Topics: Animals; Antineoplastic Agents, Phytogenic; Body Weight; Bone Marrow; Erythrocyte Count; Hematocrit; Intestine, Small; Leukocyte Count; Male; Mice; Organ Size; Sesquiterpenes; Spleen; Testis; Thymus Gland; Time Factors; Trichothecenes

Diacetoxyscirpenol (DAS, anguidine) was given intravenously to swine at 0.0, 0.5, and 1.0 mg/kg body wt. In mitotically and metabolically active tissues such as gastrointestinal epithelium and lymphoid aggregates the effects of DAS mimicked radiation poisoning. A quadratic dose-response relationship between the cytotoxicity of DAS and damage to enterocytes was found. Enterocytes in different anatomical regions of the bowel had differing susceptibilities to the toxic effects of DAS. In lymphoid tissues, DAS was preferentially cytotoxic to B-lymphocyte-rich tissues as compared to T-lymphocyte-rich tissues. In all pigs dosed with DAS the bone marrow was void of hemopoietic elements. DAS was cytotoxic to cells with specialized ion pumps, namely, renal tubular, gastric parietal, and salivary ducts. Cell damage in the exocrine and endocrine pancreas and adrenal gland accounted for changes in blood glucose. Endothelial necrosis and hemorrhage were observed in the brain. These findings were compared with those reported for other 12,13-epoxytrichothecenes and ionizing radiation and we concluded that a similar mechanism of cytotoxicity could exist.

Topics: Animals; Antineoplastic Agents, Phytogenic; Blood Glucose; Body Weight; Creatinine; Digestive System; Female; Gastric Mucosa; Ileum; Injections, Intravenous; Kidney; Liver; Lymphoid Tissue; Male; Necrosis; Sesquiterpenes; Swine; Trichothecenes

White Leghorn females in egg production (36 weeks old) were fed a culture of Fusarium roseum containing 15 ppm diacetoxyscirpenol (DAS) and other unidentified toxins at culture levels of 0, 1, and 2% of the diet for 8 weeks. Following this, all hens were placed on control (0% toxins) feed for 6 weeks. Birds were inseminated weekly with .05 ml of pooled semen from males given normal diets. The F. roseum had no significant effect on body weight change or egg weights. During the initial 8 weeks, egg production was significantly depressed by both the 1 and 2% levels whereas feed consumption, fertility, and hatchability of fertile eggs was reduced only by the 2% level of F. roseum. Moreover, the majority of embryo mortality occurred prior to the 7th day of incubation. All production levels returned to normal when the toxins were removed during the final 6 weeks. In a second experiment, control (0%), .5 ppm purified DAS, and 3% F. roseum culture were fed to White Leghorn females (50 weeks old) for 4 weeks followed by a 2-week withdrawal period when all birds were given control diets. In 4 weeks, hatchability of fertile eggs was reduced 24% by DAS and 99% by the culture of F. roseum but returned to normal after the toxins were removed. Other production indices were unaffected by dietary treatment. The DAS appears to be only partially responsible for the reduced hatchability; the major toxicant has not been identified.

Topics: Animals; Body Weight; Chick Embryo; Chickens; Diet; Feeding Behavior; Female; Fertility; Fusarium; Mortality; Mycotoxins; Oviposition; Reproduction; Sesquiterpenes; Trichothecenes