novobiocin and Leukemia--Erythroblastic--Acute

novobiocin has been researched along with Leukemia--Erythroblastic--Acute* in 2 studies

Other Studies

2 other study(ies) available for novobiocin and Leukemia--Erythroblastic--Acute

Article	Year
Reduction of Thermotolerance by Heat Shock Protein 90 Inhibitors in Murine Erythroleukemia Cells. Biological & pharmaceutical bulletin, 2018, Volume: 41, Issue:9 Cells induce heat shock proteins (HSPs) against various stress. However, murine erythroleukemia (MEL) cells do not express HSP72, a heat-inducible member of HSP70 family. So, it is of interest to examine how MEL cells respond to heat stress (44°C, 30 min). Heat stress-induced apoptosis was suppressed by pretreatment of heat shock (44°C, 10 min). Such suppressive effects were maximal at 6 h after heat shock and remained up to 12 h. Interestingly, such effects of heat shock were abrogated by specific inhibitors of HSP90 such as 17-allylamino-17-demethoxygeldanamycin (17-AAG) and novobiocin. From flow cytometric analysis, it was found that MEL cells arrest in G Topics: Animals; Apoptosis; Benzoquinones; Cell Line, Tumor; Hot Temperature; HSP90 Heat-Shock Proteins; JNK Mitogen-Activated Protein Kinases; Lactams, Macrocyclic; Leukemia, Erythroblastic, Acute; Mice; Novobiocin; Thermotolerance	2018
Clusters of replicons that fire simultaneously may be organized into superloops. DNA and cell biology, 1997, Volume: 16, Issue:9 To study the relation between replicon initiation and nuclear organization of DNA, mouse erythroleukemia F4N cells were irradiated with 60Co source and the rates of initiation of DNA synthesis were determined by a sensitive assay based on the introduction of Trioxsalen cross-links in DNA in vivo and determination of the amount of short nascent DNA fragments synthesized between the cross-links. In parallel, nuclear organization of DNA was monitored using the nucleoid sedimentation technique. The results show that DNA initiation rate and relative nucleoid sedimentation change sharply and simultaneously at doses of about 1 Gy, which suggests the existence of relationship between them. This suggestion was supported by the finding, that during the after-irradiation period, first DNA organization was restored and only after this process had been completed, the restoration of replicon initiation commenced. When cells were treated with novobiocin, an agent that is known to slow down the recovery of nucleoid sedimentation rate, initiation of DNA synthesis was also postponed. A hypothesis is put forward that replicon clusters represent groups of adjacent DNA loops organized in superloop domains and that the intact superloop domain structure is necessary for activation of the cluster. Topics: Animals; DNA; DNA Damage; DNA Replication; Gamma Rays; Leukemia, Erythroblastic, Acute; Mice; Novobiocin; Nucleic Acid Conformation; Nucleic Acid Synthesis Inhibitors; Photosensitizing Agents; Replication Origin; Trioxsalen; Tumor Cells, Cultured; Ultraviolet Rays	1997

Article

Year

Reduction of Thermotolerance by Heat Shock Protein 90 Inhibitors in Murine Erythroleukemia Cells.

Biological & pharmaceutical bulletin, 2018, Volume: 41, Issue:9

Cells induce heat shock proteins (HSPs) against various stress. However, murine erythroleukemia (MEL) cells do not express HSP72, a heat-inducible member of HSP70 family. So, it is of interest to examine how MEL cells respond to heat stress (44°C, 30 min). Heat stress-induced apoptosis was suppressed by pretreatment of heat shock (44°C, 10 min). Such suppressive effects were maximal at 6 h after heat shock and remained up to 12 h. Interestingly, such effects of heat shock were abrogated by specific inhibitors of HSP90 such as 17-allylamino-17-demethoxygeldanamycin (17-AAG) and novobiocin. From flow cytometric analysis, it was found that MEL cells arrest in G

Topics: Animals; Apoptosis; Benzoquinones; Cell Line, Tumor; Hot Temperature; HSP90 Heat-Shock Proteins; JNK Mitogen-Activated Protein Kinases; Lactams, Macrocyclic; Leukemia, Erythroblastic, Acute; Mice; Novobiocin; Thermotolerance

2018

Clusters of replicons that fire simultaneously may be organized into superloops.

DNA and cell biology, 1997, Volume: 16, Issue:9

To study the relation between replicon initiation and nuclear organization of DNA, mouse erythroleukemia F4N cells were irradiated with 60Co source and the rates of initiation of DNA synthesis were determined by a sensitive assay based on the introduction of Trioxsalen cross-links in DNA in vivo and determination of the amount of short nascent DNA fragments synthesized between the cross-links. In parallel, nuclear organization of DNA was monitored using the nucleoid sedimentation technique. The results show that DNA initiation rate and relative nucleoid sedimentation change sharply and simultaneously at doses of about 1 Gy, which suggests the existence of relationship between them. This suggestion was supported by the finding, that during the after-irradiation period, first DNA organization was restored and only after this process had been completed, the restoration of replicon initiation commenced. When cells were treated with novobiocin, an agent that is known to slow down the recovery of nucleoid sedimentation rate, initiation of DNA synthesis was also postponed. A hypothesis is put forward that replicon clusters represent groups of adjacent DNA loops organized in superloop domains and that the intact superloop domain structure is necessary for activation of the cluster.

Topics: Animals; DNA; DNA Damage; DNA Replication; Gamma Rays; Leukemia, Erythroblastic, Acute; Mice; Novobiocin; Nucleic Acid Conformation; Nucleic Acid Synthesis Inhibitors; Photosensitizing Agents; Replication Origin; Trioxsalen; Tumor Cells, Cultured; Ultraviolet Rays

1997