norbixin has been researched along with Melanoma* in 1 studies
1 other study(ies) available for norbixin and Melanoma
| Article | Year |
|---|---|
Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies.
In this present study, the inhibitory mechanism of three selected apocarotenoids (bixin, norbixin and crocin) on the diphenolase activity of tyrosinase has been investigated. The preliminary screening results indicated that apocarotenoids inhibited tyrosinase activity in a dose-dependent manner. Kinetic analysis revealed that apocarotenoids reversibly inhibited tyrosinase activity. Analysis of fluorescence spectra showed that apocarotenoids quenched the intrinsic fluorescence intensity of the tyrosinase. Further, molecular docking results implied that apocarotenoids were allosterically bound to tyrosinase through hydrophobic interactions. The results of the in vitro studies suggested that higher concentrations of bixin and norbixin inhibited tyrosinase activity in B16F0 melanoma cells. Our results suggested that apocarotenoids could form the basis for the design of novel tyrosinase inhibitors. Topics: Allosteric Site; Animals; Carotenoids; Cell Line, Tumor; Dose-Response Relationship, Drug; Hydrophobic and Hydrophilic Interactions; Kinetics; Melanins; Melanoma; Mice; Molecular Docking Simulation; Monophenol Monooxygenase; Protein Binding; Spectrometry, Fluorescence | 2016 |