nitrophenols and Plasmacytoma

Cell lines have been established that secrete a matched set of human chimeric IgM, IgG1, IgG2, IgG3, IgG4, IgE, and IgA2 antibodies that are directed against the hapten 4-hydroxy-3-nitrophenacetyl. These chimeric antibodies secreted from mouse plasmacytoma cells behave exactly like their authentic human counterparts in SDS-PAGE analysis, binding to protein A and in a wide range of serological assays. The antibodies have been compared in their ability to bind human C1q as well as in their efficacy in mediating lysis of human erythrocytes in the presence of human complement. A major conclusion to emerge is that whereas IgG3 bound C1q better than did IgG1, the chimeric IgG1 was much more effective than all the other IgG subclasses in complement-dependent hemolysis. The IgG1 antibody was also the most effective in mediating antibody-dependent cell-mediated cytotoxicity using both human effector and human target cells. These results suggest that IgG1 might be the favoured IgG subclass for therapeutic applications.

Topics: Animals; Antibodies, Monoclonal; Antibody-Dependent Cell Cytotoxicity; Cell Line; Complement Activating Enzymes; Complement C1; Complement C1q; Complement System Proteins; DNA, Recombinant; Electrophoresis, Polyacrylamide Gel; Genes, Immunoglobulin; Glycosylation; Haptens; Hemolysis; Humans; Immunoglobulins; Mice; Nitrophenols; Phenylacetates; Plasmacytoma; Plasmids; Transfection; Tumor Cells, Cultured

Topics: Animals; Antibody Formation; Antibody Specificity; Cell Fusion; Cell Line; Genes; Haptens; Hybrid Cells; Lymphocytes; Mice; Nitrophenols; Plasmacytoma; Spleen

Topics: Adsorption; Animals; Antibodies; Antibody Specificity; Dialysis; Electrophoresis, Disc; Epitopes; Immune Sera; Immunoglobulin Fragments; Immunoglobulin G; Immunoglobulin Heavy Chains; Immunoglobulins; Lysine; Mice; Mice, Inbred BALB C; Myeloma Proteins; Nitrophenols; Plasmacytoma; Rabbits; Serum Albumin; Tritium; Ultrafiltration

Topics: Adenosine Monophosphate; Animals; Antibodies; Chemical Precipitation; Choline; Haemonchus; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin A; Intestinal Mucosa; Mice; Mice, Inbred BALB C; Myeloma Proteins; Nitrophenols; Organophosphorus Compounds; Plasmacytoma; Polysaccharides, Bacterial; Pronase; Proteins; Rabbits; Rats; Streptococcus pneumoniae; Strongyloidea; Trichostrongyloidea

Topics: Animals; Antigens; Autoradiography; Cattle; Erythrocytes; Female; Humans; Immune Adherence Reaction; Immunosuppression Therapy; Mice; Mice, Inbred BALB C; Mice, Inbred DBA; Neoplasm Transplantation; Nitrophenols; Plasmacytoma; Serum Albumin, Bovine; Serum Albumin, Radio-Iodinated; Sheep; Spleen

Tumor cell lines exposed to immunoglobulins specific for cell surface antigens developed increased cellular incorporation of [(125)I]iododeoxyuridine and [(3)H]thymidine (up to 200-fold increases over cells treated with normal rabbit immunoglobulins). Antibody-stimulated cells multiplied more rapidly and lived longer than control cells in tissue culture. These observations were made both with cells substituted with 2,4,6-trinitrophenol and purified antibody against 2,4,6-trinitrophenol, and with several cell lines and their respective whole-cell antibodies. Antibodies that were stimulatory at low concentrations were cytotoxic at high concentrations. These observations may have significance in regard to enhancing effects of antibodies on tumor cell growth in vivo.

Topics: Animals; Antibodies, Neoplasm; Carcinoma; Cell Line; Colonic Neoplasms; Cross Reactions; DNA, Neoplasm; Epitopes; Female; HeLa Cells; Humans; Idoxuridine; Iodine Radioisotopes; Laryngeal Neoplasms; Mice; Neoplasms; Nitrophenols; Plasmacytoma; Rabbits; Thymidine; Tritium

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Cell Line; Cell Membrane; Centrifugation, Density Gradient; Contact Inhibition; Cytochrome Reductases; Endoplasmic Reticulum; Epithelial Cells; Epithelium; Fibroblasts; Glucose-6-Phosphatase; Membranes; Nitrophenols; Nucleotidases; Phosphoric Monoester Hydrolases; Plasmacytoma

Topics: Aminocaproates; Animals; Antibody Specificity; Bacteriophages; Chickens; Complement System Proteins; Dinitrophenols; Erythrocytes; Female; Globulins; Haptens; Hemolytic Plaque Technique; Immunoglobulin M; Iodine Radioisotopes; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred CBA; Mice, Inbred DBA; Mice, Inbred Strains; Neutralization Tests; Nitrophenols; Plasmacytoma; Precipitin Tests; Serum Albumin, Bovine; Sheep; Species Specificity

Topics: Acetates; Animals; Antineoplastic Agents; Chemical Phenomena; Chemistry; Esters; Glucuronates; Hydrolysis; Lethal Dose 50; Methanol; Mice; Nitrogen Mustard Compounds; Nitrophenols; Phenols; Phosphoric Acids; Plasmacytoma; Sulfuric Acids

BALB/c mice were immunized with three A-myeloma proteins of BALB/c-2 or BALB/c origin (produced by plasmacytomas MOPC-315, MOPC-460, Adj. PC-22A). Noncross reacting antibodies were formed against Proteins-315 and 460, but the response to Protein-22A was marginal. Proteins-315 and 460 have anti-dinitrophenyl activity, and their reactions with the corresponding BALB/c antibodies were inhibited by dinitrophenyl ligands. It appears that antibodies can be formed in BALB/c mice against unique "idiotypic" determinants in the ligand-binding sites of some BALB/c mycloma proteins.

Topics: Animals; Antigen-Antibody Reactions; Autoantibodies; Binding Sites; Epitopes; Goats; Haptens; Immune Sera; Immunoglobulin G; Iodine Isotopes; Mice; Mice, Inbred Strains; Myeloma Proteins; Neoplasms, Experimental; Nitrophenols; Plasmacytoma; Protein Binding; Radioimmunoassay; Species Specificity

Topics: Amino Acids; Animals; Binding Sites; Boron Compounds; Caproates; Chromatography; Chromatography, Gel; Diazonium Compounds; gamma-Globulins; Immunoelectrophoresis; Kinetics; Mice; Neoplasm Proteins; Neoplasms, Experimental; Nitrobenzenes; Nitrophenols; Peptides; Plasmacytoma; Protein Binding; Rabbits; Spectrophotometry; Tritium; Trypsin; Tyrosine; Ultracentrifugation