nitrophenols and Lymphoma--Follicular

Treatment response of follicular lymphomas (FL) is highly variable. We, therefore, investigated the role of FL cancer-associated fibroblasts (CAFs) on tumor cell viability, in particular in response to treatment with cytotoxic drugs. Stromal cells outgrown from FL patients were characterized and pure CAF populations were co-cultivated with FL cells. To analyze fibroblast-mediated effects, cells in co-culture were treated with ABT-737 and Bortezomib. The adherent cell population was positive for all fibroblastic markers tested and showed increased mRNA-expression of the activation marker FAP. No effect on FL cell viability was noted when co-cultivating them with CAFs. However, stromal cells protected tumor cells from apoptosis in response to cytotoxic treatment. This might be explained by mRNA-induction of ABCC1 and ABCG2 and up-regulation of BCL2L1 in FL cells. Our finding of protective mechanisms mediated by CAFs is of pivotal impact for further studies of cytotoxic agents in FL.

Topics: Antigens, CD19; Antigens, CD20; Antineoplastic Agents; Apoptosis; ATP Binding Cassette Transporter, Subfamily G, Member 2; bcl-X Protein; Biomarkers; Biphenyl Compounds; Cancer-Associated Fibroblasts; Cell Line, Tumor; Cell Survival; Coculture Techniques; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Lymphoma, Follicular; Multidrug Resistance-Associated Proteins; Neoplasm Grading; Neprilysin; Nitrophenols; Piperazines; Sulfonamides