nitrophenols and Carcinoma--Ehrlich-Tumor

The initial rate of incorporation of 14C or 3H-labeled choline into Ehrlich-Lettre ascites cells of the glycogen-free strain seven days after inoculation was investigated in vitro. 1. At choline concentrations in the medium between 6 to 30 muM and 100 to 500 muM the choline uptake by the cells followed Michaelis-Menton Kinetics with V values between 31 to 100 and 59 to 500 pmol per minute at a given cell density, and average Q10-values of 2.1 at the high and of 2.4 at the low choline molarity. The K-m-values increased from 27 muM to 58.8 muM at low and from 0.11 mM to 0.22 mM at high choline concentrations over a temperature range between 15 degrees C and 37 degrees C. Arrhenius plot of the V values gave two lines, one with a transition temperature at 25 degrees C at low and one straight line at high choline concentrations, from which the energy of activation for choline uptake was determined to be 16 kcal/mol. 2. It is assumed that two systems exist for the choline uptake by the ascites cells. One, operative at low substrate concentrations, which is saturable and probably is to be classified as a carrier-mediated facilitated diffusion process, can be strongly inhibited by deoxyglucose or 2,4-dinitrophenol and also by substrate analogues such as chlorocholine or benzoylcholine. Ouabain affects this system to a lesser extent. The other system functioning at high choline concentrations may be a simple diffusion process, which is little inhibited by substrate analogues, ouabain and deoxyglucose; however, it is also inhibited by 2,4-dinitrophenol and p-chloromercuribenzoate. 3. Choline incorporation into the acid-insoluble material (lecithin) gave linear Michaelis-Menton kinetics at the low and the high substrate concentration respectively. K-m-values decreased with an increase in temperature at low and increased with rising temperature at high substrate concentrations thus reflecting a close relationship between choline uptake and its metabolism. Labeling of lecithin choline in the various subcellular fractions under the conditions of the functioning of a carrier-mediated process was in the order: mitochondria (50%) greater than plasma membranes (25%) greater nuclei (14%) greater than microsomes (9%) greater than supernatant (1.5%). 4. Treatment of the cells with p-chloromercuribenzoate or heat shock at 50 degrees C markedly reduced the cholinee uptake and concomitantly its conversion into lecithin. Kinetic analysis revealed that the inhibitory effect of p-c

Topics: Animals; Benzoates; Carcinoma, Ehrlich Tumor; Cell Fractionation; Cell Membrane; Chlorine; Chloromercuribenzoates; Choline; Deoxyglucose; Nitrophenols; Ouabain; Phosphatidylcholines; Temperature; Thermodynamics

PS-K, a protein-bound polysaccharide from a basidiomycetes, was found to suppress tumor growth after grafting of sarcoma-180 or Ehrlich tumor in ICR mice. In the present study, effect of PS-K on antibody-forming capacities was examined in tumor-bearing mice and normal controls. 1) PS-K did not enhance the capacities of normal mice to produce antibodies against sheep erythrocytes (SRBC), hamster erythrocytes (HRBC), and trinitrophenyl group (TNP). 2) The capacities of mice to produce IgG antibody against SRBC, IgM antibody aganist HRBC, and IgG antibody against TNP were depressed after grafting of sarcoma-180. Intraperitoneal injection of PS-K restored these capacities to the normal levels. 3) Oral as well as intraperitoneal administration of PS-K restored the capacity of the mice bearing sarcoma-180 to produce IgG antibody against SRBC. 4) The capacity to produce IgG antibody against SRBC was depressed after grafting of Ehrlick tumor and it recovered to the normal level after intraperitoneal injection of PS-K. These results showed that antibody-fforming capacity of mice was depressed after tumor grafing and recovered after administration of PS-K.

Topics: Animals; Antibody Formation; Basidiomycota; Carcinoma, Ehrlich Tumor; Cricetinae; Erythrocytes; Female; Glycoproteins; Immunoglobulin G; Immunoglobulin M; Immunosuppression Therapy; Mice; Mice, Inbred ICR; Neoplasm Transplantation; Nitrophenols; Sarcoma 180

Topics: Adenosine Triphosphate; Animals; Antimycin A; Benzene Derivatives; Calcium; Carcinoma, Ehrlich Tumor; Cell Membrane Permeability; Cell Survival; Cytological Techniques; In Vitro Techniques; Iodoacetates; Ions; Magnesium; Mercury; Microscopy, Electron; Mitochondria; Mitochondrial Swelling; Nitrophenols; Potassium; Sodium; Spectrophotometry, Atomic; Staining and Labeling; Sulfonic Acids; Uncoupling Agents; Water; Water-Electrolyte Balance

Topics: Acetates; Acetylcholine; Adenosine Triphosphate; Amino Alcohols; Ammonium Sulfate; Animals; Benzoates; Brain Chemistry; Butyrates; Carcinoma, Ehrlich Tumor; Carnitine; Chemical Precipitation; Choline; Chromatography, Ion Exchange; Chromatography, Thin Layer; Cytosine Nucleotides; Hydrolysis; Insecticides; Male; Methods; Mice; Mice, Inbred Strains; Microchemistry; Nitrophenols; Organophosphorus Compounds; Phosphoric Acids; Phosphorus Isotopes; Phosphotransferases; Rats; Rats, Inbred Strains; Succinylcholine

Topics: Animals; Autoradiography; Carcinoma, Ehrlich Tumor; Dactinomycin; Depression, Chemical; Electrophoresis; Hydrogen-Ion Concentration; Metabolism; Methods; Mice; Nitrophenols; Nucleotides; Phosphates; Phosphorus Isotopes; Ribosomes; RNA, Neoplasm

Topics: Animals; Carcinoma; Carcinoma, Ehrlich Tumor; Dicumarol; Glucosamine; Glucose; Metabolism; Nitrophenols; Phenazines

Topics: 2,4-Dinitrophenol; Animals; Carbohydrate Metabolism; Carcinoma, Ehrlich Tumor; Hexoses; Neoplasms; Nitrophenols; Sodium Azide

Topics: Animals; Carbohydrate Metabolism; Carbohydrates; Carcinoma, Ehrlich Tumor; Cell Respiration; Glycolysis; Iodoacetates; Neoplasms; Nitrophenols; Respiration

Topics: Animals; Carcinoma, Ehrlich Tumor; Neoplasms, Experimental; Nitrophenols; Organophosphorus Compounds; Paraoxon; Phosphates

Topics: Animals; Carcinoma, Ehrlich Tumor; Cell Division; Dinitrophenols; Neoplasms; Nitrophenols