nitrocefin has been researched along with Mastitis--Bovine* in 2 studies
2 other study(ies) available for nitrocefin and Mastitis--Bovine
| Article | Year |
|---|---|
Comparison of tests for detection of beta-lactamase-producing staphylococci.
Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of beta-lactamase production or the beta-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use. Topics: Animals; beta-Lactamases; Cattle; Cattle Diseases; Cephalosporins; Coagulase; Colorimetry; Indicators and Reagents; Mastitis, Bovine; Microbial Sensitivity Tests; Polymerase Chain Reaction; Sensitivity and Specificity; Staphylococcal Infections; Staphylococcus; Staphylococcus aureus | 2007 |
Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection.
Resistance of Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151 S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory concentration (MIC) by a standard agar dilution technique and direct testing of beta-lactamase production using a chromogenic cephalosporin, nitrocefin. The results from these tests were compared with the presence of the beta-lactamase (blaZ) gene in the isolates, which was detected by polymerase chain reaction (PCR). Testing beta-lactamase production with nitrocefin was more predictive for the presence of the blaZ gene than the agar dilution method and the results of the former agreed highly with the presence of the blaZ gene in the isolates. In contrast, the resistance breakpoint generally used in the agar dilution method may be too high for prediction of penicillin resistance in S. aureus isolates with borderline MICs. Using this method, 40% of the isolates possessing the blaZ gene were classified as susceptible; however, majority of these isolates produced beta-lactamase when tested with nitrocefin. Topics: Animals; Anti-Bacterial Agents; beta-Lactamases; Cattle; Cephalosporins; DNA, Bacterial; Female; Mastitis, Bovine; Microbial Sensitivity Tests; Penicillin G; Penicillin Resistance; Polymerase Chain Reaction; Staphylococcal Infections; Staphylococcus aureus; Statistics, Nonparametric | 2005 |