nitroarginine has been researched along with Neuroblastoma* in 2 studies
2 other study(ies) available for nitroarginine and Neuroblastoma
| Article | Year |
|---|---|
Production of an EDRF-like activity in the cytosol of N1E-115 neuroblastoma cells.
Accumulation of cyclic GMP in cultured rat lung fibroblasts was used to test the hypothesis that N1E-115 neuroblastoma cells produce an endothelium-derived relaxing factor (EDRF)-like activity. By using this assay, the production of an EDRF-like activity in homogenates and cytosolic fractions of N1E-115 neuroblastoma cells was observed. Detection of the activity required the presence of superoxide dismutase and was inhibited by hemoglobin. Production of the EDRF-like factor was dependent on L-arginine and NADPH. The apparent Km for L-arginine was 1.25 microM and the apparent Km for NADPH was 1.67 microM. The production of the EDRF-like activity was inhibited by the L-arginine analogs, NG-monomethyl-L-arginine and NG-nitro-L-arginine, with apparent Ki values of 1.0 and 0.3 microM, respectively. Topics: Animals; Arginine; Cell-Free System; Cyclic GMP; Cytosol; Kinetics; Mice; Neuroblastoma; Nitric Oxide; Nitroarginine; omega-N-Methylarginine; Tumor Cells, Cultured | 1990 |
The cytosol of N1E-115 neuroblastoma cells synthesizes an EDRF-like substance that relaxes rabbit aorta.
The cytosolic fraction of N1E-115 neuroblastoma cells catalysed the L-arginine- and NADPH-dependent formation of a substance that relaxed endothelium-denuded strips of rabbit aorta. Relaxations in response to this substance were enhanced in the presence of superoxide dismutase. N omega-Nitro-L-arginine and NG-monomethyl-L-arginine, two inhibitors of EDRF synthesis, markedly attenuated the relaxations. Hemoglobin, a scavenger of EDRF, and methylene blue, an inhibitor of soluble guanylate cyclase, completely abolished the relaxation to N1E-115 cytosol. In contrast, the cyclo-oxygenase inhibitor indomethacin did not alter the relaxations. These data demonstrate that the cytosol of a neuronally-derived cell line is able to synthesize a substance with pharmacological properties similar to EDRF. Topics: Animals; Aorta, Thoracic; Arginine; Cytosol; Female; In Vitro Techniques; Muscle Relaxation; Muscle, Smooth, Vascular; Neuroblastoma; Nitric Oxide; Nitroarginine; omega-N-Methylarginine; Rabbits; Superoxide Dismutase; Tumor Cells, Cultured | 1989 |