nitroarginine and Intestinal-Diseases

We investigated the inhibitory synaptic potentials (ISP) in isolated smooth muscle strips of the human duodenum circular layer from the ulcer adjacent region (I group) as well as ileum and distal part of small intestine, which were on a distance of some dozen centimeters from the place of disturbance under the different gastrointestinal diseases (II group). ISP amplitude was several times smaller in the muscle strips of the I group compare to the II group. It could depend on the alterations of smooth muscles cable properties, increase of connective tissue mass, changes in the intestinal nervous system and synaptic transmission in the region adjacent to duodenum ulcer. Pyridoxal-5'-phosphate effectively decreased amplitude and increased ISP latent period in the muscle strips from both groups. N(omega)-nitro-L-arginine, the blocker of NO-synthase did not affect pyridoxal-5'-phosphate activity in smooth muscles. Phosphate group was essential for realization of its influence on ISP in smooth muscles because pyridoxal did not influence both ISP amplitude and ISP latent period.

Topics: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Enzyme Inhibitors; Humans; In Vitro Techniques; Infant; Intestinal Diseases; Intestine, Small; Middle Aged; Muscle, Smooth; Nitric Oxide Synthase; Nitroarginine; Pyridoxal Phosphate; Synaptic Transmission

The aims of this study were: 1) to obtain an experimental model reproducing the characteristics of chronicity and spontaneous relapses found in inflammatory bowel disease (IBD) and 2) to correlate these changes with intestinal motility and bacteria translocation. For this purpose, two groups of Sprague-Dawley rats were used: a treated group that received two subcutaneous injections of indomethacin (7.5 mg/kg) 48 h apart and a control group that received saline. Blood leukocytes, TNF, and fecal parameters were monitored for 90 days after treatment. In treated rats, a cyclic oscillation of blood leukocytes and TNF concomitant with an inverse correlation of fecal output was observed. Treated rats were then selected either during their highest or lowest blood leukocyte values for motor activity and microbiological evaluation. Controls were obtained in age-matched rats. Rats with high leukocyte levels showed a decrease of motor activity. In contrast, animals with low leukocyte levels presented hypermotility. Bacterial overgrowth accompanied by bacterial translocation was found in the group with high leukocytes, whereas no differences were observed between the control and indomethacin groups during the lowest leukocyte phase. We obtained a model of IBD characterized by a chronic cyclic oscillation of intestinal motility, flora, and inflammatory blood parameters. During the high-leukocyte stage, motor activity decrease is related to bacterial translocation. This phase is followed by a reactive one characterized by hypermotility associated with a decrease in both bacterial growth and leukocytes. However, as in IBD, this reaction seems unable to prevent a return to relapse.

Topics: Acute Disease; Animals; Anti-Inflammatory Agents, Non-Steroidal; Bacteria; Bacterial Infections; Bacterial Translocation; Cholecystokinin; Chronic Disease; Enteritis; Enzyme Inhibitors; Gastrointestinal Motility; Indomethacin; Injections, Subcutaneous; Intestinal Diseases; Leukocyte Count; Male; Nitroarginine; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha