nicotinamide-beta-riboside and Chemical-and-Drug-Induced-Liver-Injury

Nicotinamide riboside (NR) is a nicotinamide adenine dinucleotide (NAD. We fed C57BL/6 J mice with Lieber-DeCarli ethanol liquid diet with or without 400 mg/kg·bw NR for 16 days. Liver injuries and SirT1-PGC-1α-mitochondrial function were analyzed. In in vitro experiments, HepG2 cells (CYP2E1 over-expressing cells) were incubated with ethanol ± 0.5 mmol/L NR. Lipid accumulation and mitochondrial function were compared. SirT1 knockdown in HepG2 cells were further applied to confirm the role of SirT1 in the protection of NR on lipid accumulation.. We found that ethanol significantly decreased the expression and activity of hepatic SirT1 and induced abnormal expression of enzymes of lipid metabolism in mice. Both in vivo and in vitro experiments showed that NR activated SirT1 through increasing NAD. NR can protect against ethanol induced liver injuries via replenishing NAD

Topics: Animals; Chemical and Drug Induced Liver Injury; Ethanol; Gene Expression Regulation; Hep G2 Cells; Humans; Lipid Metabolism; Mice; Mitochondria; NAD; Niacinamide; Oxidative Stress; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Pyridinium Compounds; RNA, Long Noncoding