nickel-ferrite and Neoplasms

nickel-ferrite has been researched along with Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for nickel-ferrite and Neoplasms

ArticleYear
MMP-2-responsive fluorescent nanoprobes for enhanced selectivity of tumor cell uptake and imaging.
    Biomaterials science, 2018, Sep-25, Volume: 6, Issue:10

    It is difficult to develop highly selective substrate-based fluorescent nanoprobes for specific matrix metalloproteinases (MMPs) due to overlapping substrate specificities among the family of MMP enzymes. To resolve this issue, we have developed novel fluorescent nanoprobes that are highly selective for soluble MMP-2. Herein, MMP-2-responsive nanoprobes were prepared by immobilizing fluorescent fusion proteins on nickel ferrite nanoparticles via the His-tag nickel chelation mechanism. The fusion protein consisted of a fluorescent mCherry protein with a cell penetrating peptide (CPP) moiety. An MMP-2 cleavage site was also introduced within the fusion protein, which was directly linked to the nickel ferrite nanoparticles. The selectivity of nanoprobes was modulated by hiding the cleavage site of MMP-2 substrates deeply inside the system, which could result in strong steric hindrance between the nanoprobes and MMPs, especially for membrane-tethered MMPs such as MMP-14. A cell-based assay demonstrated that the nanoprobes could only be activated by tumor cells secreting soluble MMP-2, but not membrane-tethered MMP-14. To further evaluate the contribution of the steric hindrance effect on the nanoprobes, a truncated recombinant MMP-14 was employed to confer their cleavage activity as compared to native membrane-tethered MMP-14. Furthermore, a designed probe with a diminished steric hindrance effect was proved to be activated by membrane-tethered type MMP-14. The results indicated that the design of fluorescent nanoprobes employing the steric hindrance effect can greatly enhance the selectivity of MMP-responsive nanoprobes realizing the specific detection of soluble MMP-2 in a tumor microenvironment. We believe that highly selective MMP-2-responsive fluorescent nanoprobes have broad impacts on biomedical applications including molecular imaging and labeling for tumor detection.

    Topics: Cell Line, Tumor; Cell Survival; Cell-Penetrating Peptides; Ferric Compounds; Fluorescent Dyes; Humans; Luminescent Proteins; Matrix Metalloproteinase 14; Matrix Metalloproteinase 2; Microscopy, Electron, Transmission; Nanoparticles; Neoplasms; Nickel; Recombinant Fusion Proteins; Red Fluorescent Protein

2018
Paclitaxel loaded magnetic nanocomposites with folate modified chitosan/carboxymethyl surface; a vehicle for imaging and targeted drug delivery.
    International journal of pharmaceutics, 2016, Nov-20, Volume: 513, Issue:1-2

    In this study, Paclitaxel (PTX) containing, bovine serum albumin (BSA) nanoparticles were fabricated via a simple approach. Folic acid (FA) was conjugated to chitosan (CS)/carboxymethyl cellulose (CMC) through an esterification reaction to produce BSA-CS-FA or BSA-CMC-FA conjugates. NiFe

    Topics: Antineoplastic Agents, Phytogenic; Cell Survival; Chitosan; Drug Delivery Systems; Drug Liberation; Erythrocytes; Ferric Compounds; Folic Acid; Hemolysis; Humans; Magnetic Phenomena; MCF-7 Cells; Nanocomposites; Neoplasms; Nickel; Paclitaxel; Serum Albumin, Bovine

2016