ngr-peptide and Neoplasm-Metastasis

ngr-peptide has been researched along with Neoplasm-Metastasis* in 2 studies

Other Studies

2 other study(ies) available for ngr-peptide and Neoplasm-Metastasis

Article	Year
RGD and NGR modified TRAIL protein exhibited potent anti-metastasis effects on TRAIL-insensitive cancer cells in vitro and in vivo. Amino acids, 2017, Volume: 49, Issue:5 The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be a promising anti-tumor agent since the discovery of TRAIL-mediated apoptosis specifically on cancer cells. However, TRAIL resistance of tumor cells and patients remains to be an insurmountable obstacle for its clinical application. Here, we expressed TRAIL-related recombinant protein RGD-TRAIL, TRAIL-NGR, and RGD-TRAIL-NGR by fusing tumor targeting peptides RGD and (or) NGR at the N-terminus and C-terminus, respectively, to not only induce apoptosis of cancer cells but also inhibit metastasis. The fusion proteins possessed potent cytotoxicity with approximative IC50 in H460 and A549 cells, while TRAIL-NGR and RGD-TRAIL-NGR appeared to be more effective in HT1080 and PANC-1 cells which were relatively insensitive to TRAIL. A low concentration of fusion proteins, especially RGD-TRAIL-NGR, could inhibit migration of A549 and HT1080 cells in vitro and lung metastasis in HT1080 Topics: A549 Cells; Animals; Apoptosis; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cloning, Molecular; Female; Gene Expression; Genetic Vectors; HEK293 Cells; Humans; Lung Neoplasms; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Metastasis; NIH 3T3 Cells; Oligopeptides; Pichia; Recombinant Fusion Proteins; TNF-Related Apoptosis-Inducing Ligand; Xenograft Model Antitumor Assays	2017
Design, synthesis, and activity evaluation of a new 5-fluorouracil prodrug containing an Asn-Gly-Arg(NO2)COOCH3 tripeptide. Protein and peptide letters, 2012, Volume: 19, Issue:10 Aminopeptidase N (APN/CD13) of the M1 family is a broad specificity enzyme that is intimately involved in tumor angiogenesis and metastasis. Asparagine-glycine-arginine (NGR) is a tumor-homing tripeptide, which can selectively combine with APN/CD13 that is overexpressed on the surface of some tumor cells. Various anti-tumor drugs can be conjugated to NGR to improve selectivity, efficacy, and to decrease drug toxicity. In this study, a tripeptide NGR(NO2) was synthesized and conjugated with 5-fluorouracil. The anti-tumor activities of this new prodrug were evaluated in vivo. More significant anti-tumor effects were observed over the parent 5-fluorouracil. Topics: Animals; Antimetabolites, Antineoplastic; CD13 Antigens; Cell Movement; Cells, Cultured; Collagen; Drug Combinations; Drug Design; Female; Fluorouracil; Human Umbilical Vein Endothelial Cells; Humans; Laminin; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Metastasis; Neovascularization, Pathologic; Oligopeptides; Prodrugs; Proteoglycans; Sarcoma 180; Xenograft Model Antitumor Assays	2012

Article

Year

RGD and NGR modified TRAIL protein exhibited potent anti-metastasis effects on TRAIL-insensitive cancer cells in vitro and in vivo.

Amino acids, 2017, Volume: 49, Issue:5

The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be a promising anti-tumor agent since the discovery of TRAIL-mediated apoptosis specifically on cancer cells. However, TRAIL resistance of tumor cells and patients remains to be an insurmountable obstacle for its clinical application. Here, we expressed TRAIL-related recombinant protein RGD-TRAIL, TRAIL-NGR, and RGD-TRAIL-NGR by fusing tumor targeting peptides RGD and (or) NGR at the N-terminus and C-terminus, respectively, to not only induce apoptosis of cancer cells but also inhibit metastasis. The fusion proteins possessed potent cytotoxicity with approximative IC50 in H460 and A549 cells, while TRAIL-NGR and RGD-TRAIL-NGR appeared to be more effective in HT1080 and PANC-1 cells which were relatively insensitive to TRAIL. A low concentration of fusion proteins, especially RGD-TRAIL-NGR, could inhibit migration of A549 and HT1080 cells in vitro and lung metastasis in HT1080

Topics: A549 Cells; Animals; Apoptosis; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cloning, Molecular; Female; Gene Expression; Genetic Vectors; HEK293 Cells; Humans; Lung Neoplasms; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Metastasis; NIH 3T3 Cells; Oligopeptides; Pichia; Recombinant Fusion Proteins; TNF-Related Apoptosis-Inducing Ligand; Xenograft Model Antitumor Assays

2017

Design, synthesis, and activity evaluation of a new 5-fluorouracil prodrug containing an Asn-Gly-Arg(NO2)COOCH3 tripeptide.

Protein and peptide letters, 2012, Volume: 19, Issue:10

Aminopeptidase N (APN/CD13) of the M1 family is a broad specificity enzyme that is intimately involved in tumor angiogenesis and metastasis. Asparagine-glycine-arginine (NGR) is a tumor-homing tripeptide, which can selectively combine with APN/CD13 that is overexpressed on the surface of some tumor cells. Various anti-tumor drugs can be conjugated to NGR to improve selectivity, efficacy, and to decrease drug toxicity. In this study, a tripeptide NGR(NO2) was synthesized and conjugated with 5-fluorouracil. The anti-tumor activities of this new prodrug were evaluated in vivo. More significant anti-tumor effects were observed over the parent 5-fluorouracil.

Topics: Animals; Antimetabolites, Antineoplastic; CD13 Antigens; Cell Movement; Cells, Cultured; Collagen; Drug Combinations; Drug Design; Female; Fluorouracil; Human Umbilical Vein Endothelial Cells; Humans; Laminin; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Metastasis; Neovascularization, Pathologic; Oligopeptides; Prodrugs; Proteoglycans; Sarcoma 180; Xenograft Model Antitumor Assays

2012