neuropeptide-y and Leishmaniasis--Cutaneous

Neuropeptide Y (NPY) concentrations were investigated by radioimmunoassay and immunohistochemistry in a murine model of cutaneous leishmaniasis, using a susceptible (BALB/c) and a resistant (C57BL/6) mouse strain. The analyses were performed on the skin, secondary lymphoid organs and dorsal root ganglia (DRG) at 1, 3, 6 and 9 weeks postinfection. An overall reduction in the NPY concentrations in the studied organs was observed in both mouse strains; the reduction in the skin and draining lymph nodes was more evident and progressive in the susceptible strain. Using immunohistochemistry there seemed to be a reduction in NPY immunoreactivity in all inflamed tissues analysed compared to the controls. These observations might indicate a possible pathophysiological role for NPY in murine cutaneous leishmaniasis.

Topics: Animals; Chromatography, High Pressure Liquid; Female; Ganglia, Spinal; Leishmania; Leishmaniasis, Cutaneous; Lymph Nodes; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Neuropeptide Y; Skin; Species Specificity; Spleen