neuropeptide-y and Growth-Disorders

The possible role of neuropeptide Y (NPY) in mediating the relationship between pituitary LH secretion and growth retardation due to restricted feeding was examined in ovariectomized (OVX) prepubertal ewe lambs. One specific objective examined whether there was an inverse relationship between concentrations of NPY in four diencephalic brain regions and pituitary LH secretion in ewe lambs 29-30 weeks old which had been growth retarded since 8 weeks and OVX at 24 weeks. Through dietary restriction, body weight was held constant at 20.7 +/- 0.5 kg in 13 growth-retarded ewes as compared with 48.0 +/- 0.6 kg for 5 age-matched control ewes at 29-30 weeks of age. Episodic LH was quantified at 10-min intervals for 190 min/day on 3 of the 8 days immediately before euthanasia. Serum LH averaged 6.5 +/- 0.6 ng/ml in control ewes with a mean pulse frequency of 1.0 +/- 0.1 pulses/h. Serum LH in growth-retarded ewes was much less episodic (0.2 +/- 0.05 pulses/h) and averaged only 1.2 +/- 0.2 ng/ml. All ewes were euthanized during week 30, and the following brain regions were dissected: basal forebrain, preoptic area, median eminence and remainder of hypothalamus. Following extraction, NPY concentrations (pg/mg of original tissue) were quantified by radioimmunoassay. In each brain region, NPY concentrations were greater (p < 0.05) in 6 growth-retarded ewes than in 5 control ewes (median eminence: 5.2 vs. 0.6; remainder of hypothalamus: 3.3 vs. 0.8; preoptic area: 3.1 vs. 0.8, and basal forebrain: 2.2 vs. 1.2). A secondary objective examined whether the LH and NPY parameters were rapidly altered by transient changes in feed consumption.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Animals; Female; Food Deprivation; Gonadotropin-Releasing Hormone; Growth Disorders; Hypothalamus; Luteinizing Hormone; Neuropeptide Y; Ovariectomy; Ovary; Pituitary Gland; Secretory Rate; Sheep