neuropeptide-y--leu(31)-pro(34)- has been researched along with Pheochromocytoma* in 1 studies
1 other study(ies) available for neuropeptide-y--leu(31)-pro(34)- and Pheochromocytoma
Article | Year |
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Neuropeptide Y inhibits depolarization-stimulated catecholamine synthesis in rat pheochromocytoma cells.
In PC12 rat pheochromocytoma cells differentiated with nerve growth factor (NGF), neuropeptide Y inhibited depolarization-stimulated catecholamine synthesis as determined by in situ measurement of 3,4-dihydroxyphenylalanine (DOPA) production in the presence of the decarboxylase inhibitor m-hydroxybenzylhydrazine (NSD-1015). The inhibition by neuropeptide Y was concentration-dependent and was prevented by pretreatment with pertussis toxin, suggesting the involvement of a GTP-binding protein of the Gi or Go subtype. The neuropeptide Y analog [Leu31,Pro34]neuropeptide Y also caused inhibition of DOPA production, but was less potent than neuropeptide Y itself, while peptide YY and neuropeptide Y-(13-36) had no significant effect. This pattern is most consistent with the involvement of the neuropeptide Y Y3 receptor subtype. In PC12 cells differentiated with dexamethasone, neuropeptide Y also caused a concentration-dependent inhibition of DOPA production, while peptide YY was again without effect. Neuropeptide Y had no effect on DOPA production in undifferentiated PC12 cells. These results indicate that neuropeptide Y can modulate catecholamine synthesis in addition to its modulatory effects on catecholamine release. Topics: Adrenal Gland Neoplasms; Analysis of Variance; Animals; Anti-Inflammatory Agents, Non-Steroidal; Aromatic Amino Acid Decarboxylase Inhibitors; Cell Differentiation; Dexamethasone; Dihydroxyphenylalanine; Dose-Response Relationship, Drug; Gastrointestinal Hormones; GTP-Binding Proteins; Hydrazines; Nerve Growth Factors; Neuropeptide Y; PC12 Cells; Peptide Fragments; Peptide YY; Peptides; Pertussis Toxin; Pheochromocytoma; Rats; Structure-Activity Relationship; Virulence Factors, Bordetella | 1995 |