neuromedin-b and Pain

neuromedin-b has been researched along with Pain* in 3 studies

Other Studies

3 other study(ies) available for neuromedin-b and Pain

ArticleYear
Functional roles of neuromedin B and gastrin-releasing peptide in regulating itch and pain in the spinal cord of non-human primates.
    Biochemical pharmacology, 2022, Volume: 198

    Despite accumulating evidence in rodents, the functional role of neuromedin B (NMB) in regulating somatosensory systems in primate spinal cord is unknown. We aimed to compare the expression patterns of NMB and its receptor (NMBR) and the behavioral effects of intrathecal (i.t.) NMB with gastrin-releasing peptide (GRP) on itch or pain in non-human primates (NHPs). We used six adult rhesus monkeys. The mRNA or protein expressions of NMB, GRP, and their receptors were evaluated by quantitative reverse transcription polymerase chain reaction, immunohistochemistry, or in situ hybridization. We determined the behavioral effects of NMB or GRP via acute thermal nociception, capsaicin-induced thermal allodynia, and itch scratching response assays. NMB expression levels were greater than those of GRP in the dorsal root ganglia and spinal dorsal horn. Conversely, NMBR expression was significantly lower than GRP receptor (GRPR). I.t. NMB elicited only mild scratching responses, whereas GRP caused robust scratching responses. GRP- and NMB-elicited scratching responses were attenuated by GRPR (RC-3095) and NMBR (PD168368) antagonists, respectively. Moreover, i.t. NMB and GRP did not induce thermal hypersensitivity and GRPR and NMBR antagonists did not affect peripherally elicited thermal allodynia. Consistently, NMBR expression was low in both itch- and pain-responsive neurons in the spinal dorsal horn. Spinal NMB-NMBR system plays a minimal functional role in the neurotransmission of itch and pain in primates. Unlike the functional significance of the GRP-GRPR system in itch, drugs targeting the spinal NMB-NMBR system may not effectively alleviate non-NMBR-mediated itch.

    Topics: Animals; Gastrin-Releasing Peptide; Hyperalgesia; Neurokinin B; Pain; Primates; Pruritus; Receptors, Bombesin; Spinal Cord; Spinal Cord Dorsal Horn

2022
Neuromedin B receptor stimulation of Cav3.2 T-type Ca
    Theranostics, 2021, Volume: 11, Issue:19

    Topics: Action Potentials; Animals; Calcium Channels, T-Type; Cyclic AMP-Dependent Protein Kinases; Female; Freund's Adjuvant; Ganglia, Spinal; Male; Mice; Mice, Inbred ICR; Neurokinin B; Pain; Receptors, Bombesin; Receptors, G-Protein-Coupled; Sensory Receptor Cells; Signal Transduction; Trigeminal Ganglion

2021
The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons.
    Molecular pain, 2012, Jul-09, Volume: 8

    Itch is one of the major somatosensory modalities. Some recent findings have proposed that gastrin releasing peptide (Grp) is expressed in a subset of dorsal root ganglion (DRG) neurons and functions as a selective neurotransmitter for transferring itch information to spinal cord interneurons. However, expression data from public databases and earlier literatures indicate that Grp mRNA is only detected in dorsal spinal cord (dSC) whereas its family member neuromedin B (Nmb) is highly expressed in DRG neurons. These contradictory results argue that a thorough characterization of the expression of Grp and Nmb is warranted.. Grp mRNA is highly expressed in dSC but is barely detectable in DRGs of juvenile and adult mice. Anti-bombesin serum specifically recognizes Grp but not Nmb. Grp is present in a small number of small-diameter DRG neurons and in abundance in layers I and II of the spinal cord. The reduction of dSC Grp after dorsal root rhizotomy is significantly different from those of DRG derived markers but similar to that of a spinal cord neuronal marker. Double fluorescent in situ of Nmb and other molecular markers indicate that Nmb is highly and selectively expressed in nociceptive and itch-sensitive DRG neurons.. The majority of dSC Grp is synthesized locally in dorsal spinal cord neurons. On the other hand, Nmb is highly expressed in pain- and itch-sensing DRG neurons. Our findings provide direct anatomic evidence that Grp could function locally in the dorsal spinal cord in addition to its roles in DRG neurons and that Nmb has potential roles in nociceptive and itch-sensitive neurons. These results will improve our understanding about roles of Grp and Nmb in mediating itch sensation.

    Topics: Aging; Amino Acid Sequence; Animals; Antibody Specificity; Bombesin; Cold Temperature; Ganglia, Spinal; Gastrin-Releasing Peptide; Gene Expression Regulation, Developmental; Humans; Immune Sera; Mechanotransduction, Cellular; Mice; Molecular Sequence Data; Neurokinin B; Nociceptors; Pain; Pain Threshold; Physical Stimulation; Protein Transport; Pruritus; Receptors, Bombesin; Rhizotomy; RNA, Messenger; Sensory Receptor Cells; Spinal Cord

2012