negamycin and Body-Weight

negamycin has been researched along with Body-Weight* in 1 studies

Other Studies

1 other study(ies) available for negamycin and Body-Weight

ArticleYear
Negamycin restores dystrophin expression in skeletal and cardiac muscles of mdx mice.
    Journal of biochemistry, 2003, Volume: 134, Issue:5

    The ability of aminoglycoside antibiotics to promote read-through of nonsense mutations has attracted interest in these drugs as potential therapeutic agents in genetic diseases. However, the toxicity of aminoglycoside antibiotics may result in severe side effects during long-term treatment. In this paper, we report that negamycin, a dipeptide antibiotic, also restores dystrophin expression in skeletal and cardiac muscles of the mdx mouse, an animal model of Duchenne muscular dystrophy (DMD) with a nonsense mutation in the dystrophin gene, and in cultured mdx myotubes. Dystrophin expression was confirmed by immunohistochemistry and immunoblotting. We also compared the toxicity of negamycin and gentamicin, and found negamycin to be less toxic. Furthermore, we demonstrate that negamycin binds to a partial sequence of the eukaryotic rRNA-decoding A-site. We conclude that negamycin is a promising new therapeutic candidate for DMD and other genetic diseases caused by nonsense mutations.

    Topics: Amino Acids, Diamino; Animals; Body Weight; Brain Stem; Dystrophin; Gene Expression Regulation; Gentamicins; Male; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Muscle, Skeletal; Muscular Dystrophy, Animal; Muscular Dystrophy, Duchenne; Myocardium; RNA, Ribosomal

2003