nefurthiazole and Body-Weight

Factors enhancing renal carcinogenesis induced by formic acid 2-[4-(5-nitro-2-furyl)-2-thiazolyl] hydrazide (FNT) were studied. Five groups of rats were used. Groups 1 and 2 were subjected to right nephrectomy and 2 days late they were injected with 20 mg/kg body weight of dimethylnitrosamine (DMN). From one week after nephrectomy, group 1 was fed on 0.2% FNT diet continuously to the end of the experiment, and group 2 was given normal diet. In week 2, they were given 400 mg/kg body weight of chloroform (CHCl3) orally. Groups 3 and 4 were given ChCl3 first and 2 days later injected with DMN. Group 3 was then fed FNT diet and group 4 was fed basal diet continuously. In week 2, groups 3 and 4 were subjected to unilateral nephrectomy. Group 5 was given FNT diet without other treatment. The incidences of tubular hyperplasia in groups 1, 3 and 5 were 53.3%, 52.9%, and 0%, respectively, at week 16 and 100%, 94.9% and 94.1%, respectively, at week 32. Moreover, the incidences of renal cell tumors in these groups were 85.0%, 88.9% and 17.6%, respectively, at week 32. Thus, the inductions of tubular hyperplasia and renal cell tumor were markedly enhanced by combined treatment with CHCl3, DMN, unilateral nephrectomy and FNT in the initiating and promoting stages.

Topics: Animals; Body Weight; Carcinogens; Chloroform; Dimethylnitrosamine; Kidney Neoplasms; Male; Neoplasms, Experimental; Nephrectomy; Nitrofurans; Organ Size; Rats; Rats, Inbred F344; Thiazoles

We gave 102 male Fischer 344 rats (100 to 200 g) 0.2 per cent nitrofurylthiazolyl formamide (FANFT) diet for 14 weeks. Half of the animals underwent urinary diversion and half, sham operation. All animals resumed the FANFT diet. Half of each of the groups received four weekly i.p. injections of cyclophosphamide and the remainder received saline injections. The animals were sacrificed 12 weeks after diversion or sham operation. The incidences of tumor and tumor invasion were tabulated. Urinary diversion produced a greater effect in reducing progression of early neoplastic lesions to gross or microscopically invasive tumor than did systemic cyclophosphamide; however, an additive effect was noted when diversion and cyclophosphamide therapy were combined.

Topics: Animals; Body Weight; Carcinogens; Carcinoma, Papillary; Cyclophosphamide; Male; Neoplasms, Experimental; Nitrofurans; Organ Size; Rats; Thiazoles; Urinary Bladder; Urinary Bladder Neoplasms; Urinary Diversion