natriuretic-peptide--brain has been researched along with Astrocytoma* in 2 studies
2 other study(ies) available for natriuretic-peptide--brain and Astrocytoma
| Article | Year |
|---|---|
Hydrolysis of atrial and brain natriuretic peptides by the human astrocytoma clone D384 and the neuroblastoma line SH-SY5Y.
The content of membrane peptidases has been compared in the human astrocytoma clone D384 and the human neuroblastoma line SH-SY5Y. Endopeptidase-24.11 (neutral endopeptidase, EC 3.4.24.11) was detectable only on the astrocytoma cells whereas angiotensin-converting enzyme (EC 3.4.15.1) was selectively expressed on the neuroblastoma line. Dipeptidyl peptidase IV (EC 3.4.14.5) was also abundant on the astrocytoma line. The presence of both endopeptidase-24.11 and dipeptidyl peptidase IV on D384 cells was confirmed by immunohistochemistry. A membrane preparation from D384 cells hydrolyzed both atrial natriuretic peptide and brain natriuretic peptide and, in both cases, the pattern of metabolism was similar to that seen with purified endopeptidase-24.11. The endopeptidase-24.11 inhibitor, phosphoramidon, at 1 microM abolished natriuretic peptide metabolism. The neuroblastoma line, which lacked endopeptidase-24.11, failed to metabolise atrial natriuretic peptide and brain natriuretic peptide, emphasizing the key role of the endopeptidase in hydrolyzing these regulatory peptides at the cell surface. Topics: Astrocytoma; Atrial Natriuretic Factor; Cell Membrane; Dipeptidyl Peptidase 4; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; Fluorescent Antibody Technique; Humans; Hydrolysis; Natriuretic Peptide, Brain; Neprilysin; Nerve Tissue Proteins; Neuroblastoma; Peptidyl-Dipeptidase A; Tumor Cells, Cultured | 1991 |
Hydrolysis of natriuretic peptides by the human astrocytoma clone D384.
Topics: Astrocytoma; Atrial Natriuretic Factor; Cell Line; Cell Membrane; Clone Cells; Endopeptidases; Humans; Hydrolysis; Natriuretic Peptide, Brain; Nerve Tissue Proteins; Neuroblastoma | 1990 |