naphthoquinones and Peritonitis

LQB 118, a hydride molecule, has been described as an antineoplastic and antiparasitic drug. Recently, LQB118 was also shown to display anti-inflammatory properties using an LPS-induced lung inflammation model. However, LQB 118 effects on the inflammatory response induced by zymosan has not been demonstrated. In this study, swiss mice were LQB 118 intraperitoneally (i.p.) treated and zymosan was used to induce peritoneal inflammation. Peritoneal fluid was collected and used for cell counting and proinflammatory cytokines quantification (IL-1β, IL-6, and TNF-α) by immunoenzymatic assay (ELISA). For in vitro studies, peritoneal macrophages zymosan-stimulated were used. Results demonstrated that LQB 118 treatment reduced polymorphonuclear cell migration and TNF-α, IL-1β, and IL-6 levels in the peritoneal cavity. In macrophages, LQB 118 treatment display no cytotoxic effect and is also able to reduce cytokines levels. To investigate LQB 118 putative mechanism of action, TLR2, CD69, and P-p38 MAPK expression were evaluated. LQB 118 treatment reduced CD69 expression and p38 phosphorylation induced by zymosan. Furthermore, LQB 118 was able to negatively modulate TLR2 expression in the presence of inflammatory stimulus. Thus, our study provide new evidences for the mechanisms related to the anti-inflammatory effect of LQB 118 in vivo and in vitro.

Topics: Animals; Anti-Inflammatory Agents; Antineoplastic Agents; Cells, Cultured; Cytokines; Disease Models, Animal; Female; Humans; Inflammation; Inflammation Mediators; Macrophages; Mice; Naphthoquinones; Peritoneum; Peritonitis; Pterocarpans; Zymosan

Topics: Animals; Anti-Bacterial Agents; Boraginaceae; Drug Synergism; Mice; Microbial Sensitivity Tests; Naphthoquinones; Pentanoic Acids; Peritonitis; Sepsis; Staphylococcal Infections; Staphylococcus aureus

Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcription factor that plays a crucial role in protection of cells from electrophile-induced toxicity through up-regulating phase II detoxifying enzymes and phase III transporters. We previously reported that oxidative stress induces up-regulation of interleukin-11 (IL-11), a member of the IL-6 family that ameliorates acetaminophen-induced liver toxicity. However, a role for IL-11 in protection of cells from electrophile-induced toxicity remains unclear. Here we show that an environmental electrophile, 1,2-naphthoquinone (1,2-NQ), but not 15d-prostaglandin J

Topics: Animals; Antineoplastic Agents; Cells, Cultured; Gene Expression Regulation; HEK293 Cells; Hep G2 Cells; Humans; Hydrogen Peroxide; Interleukin-11; Interleukin-11 Receptor alpha Subunit; Intestinal Diseases; MAP Kinase Signaling System; Mice; Mice, Inbred C57BL; Mice, Knockout; Naphthoquinones; NF-E2-Related Factor 2; Oxidants; Oxidative Stress; Peritonitis; Prostaglandin D2; Reactive Oxygen Species