naphthoquinones and Anemia--Aplastic

This study is aimed at investigating the effects of shikonin, a pyruvate kinase M2 (PKM2) inhibitor, on the functions of myeloid dendritic cells (mDCs) in a mouse model of severe aplastic anemia (AA) generated by total body irradiation and lymphocyte infusion. Flow cytometry and qPCR were used to determine the proportions of PKM2+ mDCs and other immune indicators in the AA mice. Glucose consumption level, pyruvate generation level, and ATP content were used to determine the level of glycolytic metabolism in the mDCs. The survival rates of AA mice were evaluated after the administration of shikonin or the immunosuppressive agent cyclosporin A. The AA mice displayed pancytopenia, decreased CD4+/CD8+ cell ratio, increased perforin and granzyme levels in CD8+ cells, increased costimulatory CD80 and CD86 expressions, and inadequate regulatory T cell number.

Topics: Anemia, Aplastic; Animals; B7-1 Antigen; B7-2 Antigen; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cyclosporine; Dendritic Cells; Flow Cytometry; Immunosuppressive Agents; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Naphthoquinones; Pyruvate Kinase; Reverse Transcriptase Polymerase Chain Reaction