naphthalan and Psoriasis

Immunohistochemical analysis (cell immunophenotyping before and after 3 weeks of treatment with naphthalan oil) was performed on biopsy specimens from 10 patients with psoriasis vulgaris. To determine the angiogenetic factor in psoriatic lesions, immunohistochemical staining on 3-microm paraffin block sections was performed by using monoclonal anti-factor VIII antibody. There was a significant difference in the mean number of new blood vessels before and after the therapy (15.1 vs. 6.7). It seems that naphthalan therapy reduces number of new blood vessels and has effect on neovascularization in patients with psoriasis.

Topics: Administration, Topical; Angiogenesis Inhibitors; Baths; Female; Humans; Male; Naphthalenes; Neovascularization, Physiologic; Psoriasis; Skin

Immunohistochemistry analysis by cell immunophenotyping before and after a 3 - week treatment with naphthalan oil was performed on biopsy specimens from 10 patients with psoriasis vulgaris. For immunohistochemistry staining of 3- micro m paraffin block sections, anti-CD3, anti-CD4, anti-CD8, and Ki-67 antibodies were used. Peroxidase reaction for T cell (CD3, CD4, and CD8) quantification was done in the epidermis and dermis of each skin sample as total positive cell count per mm sample. Positive Ki-67 (proliferation index) was determined as percentage of positive cells per 100 cells in the basal layer of the epidermis. Results showed naphthalan treatment to decrease the mean CD3 lymphocyte count by 83% and 59%, CD4 lymphocyte count by 81% and 73%, and CD8 lymphocyte count by 60% and 49% in the epidermis and dermis, respectively. The mean proliferation index also decreased with naphthalan therapy.

Topics: Adult; CD3 Complex; CD4 Antigens; CD8 Antigens; Cell Proliferation; Epidermal Cells; Epidermis; Female; Humans; Immunohistochemistry; Ki-67 Antigen; Lymphocyte Count; Male; Naphthalenes; Oils; Psoriasis