Compounds > n-t-butyl-n--tetradecyl-3-tetradecylaminopropionamidine

n-t-butyl-n--tetradecyl-3-tetradecylaminopropionamidine and Leukemia--Myelogenous--Chronic--BCR-ABL-Positive

n-t-butyl-n--tetradecyl-3-tetradecylaminopropionamidine has been researched along with Leukemia--Myelogenous--Chronic--BCR-ABL-Positive* in 1 studies

Other Studies

1 other study(ies) available for n-t-butyl-n--tetradecyl-3-tetradecylaminopropionamidine and Leukemia--Myelogenous--Chronic--BCR-ABL-Positive

Article	Year
A novel cationic amphiphile for transfection of mammalian cells. Biochemical and biophysical research communications, 1994, Sep-30, Volume: 203, Issue:3 We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter. Topics: Amidines; Animals; Cations; Cells, Cultured; Chloramphenicol O-Acetyltransferase; CHO Cells; Cricetinae; DNA; Humans; Indicators and Reagents; Kinetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Liposomes; Mammals; Plasmids; Time Factors; Transfection; Tumor Cells, Cultured	1994

Article

Year

A novel cationic amphiphile for transfection of mammalian cells.

Biochemical and biophysical research communications, 1994, Sep-30, Volume: 203, Issue:3

We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter.

Topics: Amidines; Animals; Cations; Cells, Cultured; Chloramphenicol O-Acetyltransferase; CHO Cells; Cricetinae; DNA; Humans; Indicators and Reagents; Kinetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Liposomes; Mammals; Plasmids; Time Factors; Transfection; Tumor Cells, Cultured

1994