n-stearoyltyrosine and Weight-Gain

N-stearoyltyrosine dipotassium (NST-2K) as a neuroprotective candidate is currently in preclinical studies in China. This study investigated the anti-obese effect of NST-2K in high-fat diet-induced obese (DIO) mice. The DIO mice were induced from male C57BL/6 mice by feeding high-fat diet for 11-weeks and treated orally with NST-2K for other 4 weeks. The treatments of DIO mice with NST-2K at 60 or 100 mg/kg/day suppressed the body weight gain, decreased both visceral fat weight and adipocyte size without influence on food intake. To evaluate the effect of NST-2K on lipid metabolism, lipid parameters and several key molecules in the plasma, liver, duodenum mucosa and adipose tissue were analyzed. NST-2K ameliorated the low-grade inflammation in liver, inhibited pancreatic lipase activity in duodenum mucosa, activated β-oxidation system and reduced lipogenesis, thus suppressed lipid accumulation in the liver, reduced adipocyte size and improved lipid and carbohydrate metabolism. Overall, without influence on food intake, NST-2K ameliorated high-fat diet-induced obesity via suppressing liver inflammation, inhibiting dietary fat absorption, promoting lipolysis and reducing lipogenesis.

Topics: Adiposity; Animals; Anti-Inflammatory Agents, Non-Steroidal; Anti-Obesity Agents; Dietary Fats; Dose-Response Relationship, Drug; Drugs, Investigational; Duodenum; Gastrointestinal Agents; Intestinal Absorption; Intestinal Mucosa; Lipase; Lipogenesis; Lipolysis; Liver; Male; Mice, Inbred C57BL; Neuroprotective Agents; Obesity; Random Allocation; Tyrosine; Weight Gain