n-oleoylethanolamine and Neuroectodermal-Tumors--Primitive--Peripheral

n-oleoylethanolamine has been researched along with Neuroectodermal-Tumors--Primitive--Peripheral* in 1 studies

Other Studies

1 other study(ies) available for n-oleoylethanolamine and Neuroectodermal-Tumors--Primitive--Peripheral

Article	Year
N-Oleoylethanolamine inhibits glucosylation of natural ceramides in CHP-100 neuroepithelioma cells: possible implications for apoptosis. Biochemical and biophysical research communications, 1999, Feb-16, Volume: 255, Issue:2 We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18h to non-toxic NOE concentrations (i.e. up to 70 microM), basal incorporation of labelled hexose into glucosylceramide (GlcCer) and higher order neutral glycosphingolipids was significantly inhibited. In cells treated with 30 microM N-hexanoylsphingosine (C6-Cer), NOE affected only marginally short-chain glucocerebroside accumulation, but markedly decreased accumulation of glucocerebrosides originating from glucosylation of a long-chain ceramide (Lc-Cer) pool produced upon C6-Cer treatment. Evidence is provided that NOE effects neither are mediated by their effects on ceramidase nor are due to enhanced long-chain GlcCer (Lc-GlcCer) conversion to higher order glycosylated derivatives. NOE inhibition of Lc-GlcCer generation was accompanied by enhanced accumulation of Lc-Cer and by potentiation of apoptosis induced by C6-Cer; the possible causal relationships between these two phenomena are discussed. Topics: Acylation; Amidohydrolases; Antigens, CD; Apoptosis; Carbon Radioisotopes; Ceramidases; Ceramides; Dose-Response Relationship, Drug; Endocannabinoids; Enzyme Inhibitors; Ethanolamines; Glycosylation; Humans; Lactosylceramides; Neuroectodermal Tumors, Primitive, Peripheral; Oleic Acids; Palmitic Acid; Tumor Cells, Cultured	1999

Article

Year

N-Oleoylethanolamine inhibits glucosylation of natural ceramides in CHP-100 neuroepithelioma cells: possible implications for apoptosis.

Biochemical and biophysical research communications, 1999, Feb-16, Volume: 255, Issue:2

We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18h to non-toxic NOE concentrations (i.e. up to 70 microM), basal incorporation of labelled hexose into glucosylceramide (GlcCer) and higher order neutral glycosphingolipids was significantly inhibited. In cells treated with 30 microM N-hexanoylsphingosine (C6-Cer), NOE affected only marginally short-chain glucocerebroside accumulation, but markedly decreased accumulation of glucocerebrosides originating from glucosylation of a long-chain ceramide (Lc-Cer) pool produced upon C6-Cer treatment. Evidence is provided that NOE effects neither are mediated by their effects on ceramidase nor are due to enhanced long-chain GlcCer (Lc-GlcCer) conversion to higher order glycosylated derivatives. NOE inhibition of Lc-GlcCer generation was accompanied by enhanced accumulation of Lc-Cer and by potentiation of apoptosis induced by C6-Cer; the possible causal relationships between these two phenomena are discussed.

Topics: Acylation; Amidohydrolases; Antigens, CD; Apoptosis; Carbon Radioisotopes; Ceramidases; Ceramides; Dose-Response Relationship, Drug; Endocannabinoids; Enzyme Inhibitors; Ethanolamines; Glycosylation; Humans; Lactosylceramides; Neuroectodermal Tumors, Primitive, Peripheral; Oleic Acids; Palmitic Acid; Tumor Cells, Cultured

1999