n-benzyloxycarbonyl-leucyl-valyl-glycine-diazomethane and Streptococcal-Infections

Streptococcus (S.) phocae subsp. phocae causes bronchopneumonia and septicemia in a variety of marine mammals. Especially in harbor seals infected with phocine distemper virus it plays an important role as an opportunistic pathogen. This study was initiated by the detection of IgG cleavage products in Western blot analysis after incubation of bacterial supernatant with harbor seal serum. Hence, the objectives of this study were the identification and characterization of a secreted IgG cleaving protease in S. phocae subsp. phocae isolated from marine mammals. To further identify the responsible factor of IgG cleavage a protease inhibitor profile was generated. Inhibition of the IgG cleaving activity by iodoacetamide and Z-LVG-CHN

Topics: Animals; Bacterial Proteins; Cross Reactions; Endopeptidases; Host Specificity; Immune Evasion; Immunoglobulin G; Iodoacetamide; Oligopeptides; Phoca; Protease Inhibitors; Recombinant Proteins; Sequence Analysis, DNA; Streptococcal Infections; Streptococcus pyogenes