n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride and Neoplasms

n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride has been researched along with Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride and Neoplasms

ArticleYear
Safe and effective delivery of small interfering RNA with polymer- and liposomes-based complexes.
    Biological & pharmaceutical bulletin, 2013, Volume: 36, Issue:6

    We developed binary and ternary complexes based on polymers and liposomes for safe and effective delivery of small interfering RNA (siRNA). Anti-luciferase siRNA was used as a model of nucleic acid medicine. The binary complexes of siRNA were prepared with cationic polymers and cationic liposomes such as polyethylenimine (PEI), polyamidoamine (PAMAM) dendrimer, poly-L-arginine (PLA), trimethyl[2,3-(dioleoxy)-propyl]ammonium chloride (DOTMA), and cholesteryl 3β-N-(dimetylaminnoethyl)carbamate hydrochloride (DC-Chol). The ternary complexes were constructed by the addition of γ-polyglutamic acid (γ-PGA) to the binary complexes. The complexes were approximately 54-153 nm in particle size. The binary complexes showed a cationic surface charge although an anionic surface charge was observed in the ternary complexes. The polymer-based complexes did not show a silencing effect in the mouse colon carcinoma cell line expressing luciferase regularly (Colon26/Luc cells). The binary complexes based on liposomes and their ternary complexes coated by γ-PGA showed a significant silencing effect. The binary complexes showed significant cytotoxicity although the ternary complexes coated by γ-PGA did not show significant cytotoxicity. The ternary complexes coated by γ-PGA suppressed luciferase activity in the tumor after their direct injection into the tumors of mice bearing Colon26/Luc cells. Thus, we have newly identified safe and efficient ternary complexes of siRNA for clinical use.

    Topics: Animals; Cell Line, Tumor; Cholesterol; Female; Gene Silencing; Liposomes; Luciferases; Mice; Mice, Inbred BALB C; Neoplasms; Polymers; Quaternary Ammonium Compounds; RNA, Small Interfering

2013