Compounds > n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride

n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride and Adenocarcinoma

n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride has been researched along with Adenocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for n-(1-(2-3-dioleyloxy)propyl)-n-n-n-trimethylammonium-chloride and Adenocarcinoma

Article	Year
Cationic lipids improve antisense oligonucleotide uptake and prevent degradation in cultured cells and in human serum. Biochemical and biophysical research communications, 1993, Dec-15, Volume: 197, Issue:2 The power of antisense phosphodiester oligonucleotides (aODN) as regulatory molecules of gene expression is strongly limited by their low cellular uptake and very rapid nuclease-mediated degradation. This study deals with the effect of artificial cationic lipids on ODN cellular uptake and degradation in cell cultures and in human serum. At the ODN levels normally used in antisense-mediated gene regulation experiments, a cationic lipid, DOTAP, enhances the rate of ODN uptake more than 25 fold, but at lower ODN levels the effect of DOTAP is absent. These findings are consistent with a mechanism of ODN internalization by receptor-mediated saturable endocytosis that is bypassed by DOTAP. ODN degradation by nucleases is markedly prevented by DOTAP both in cultured cells and in human serum. Other cationic lipids, namely DOTMA and DOGS, exhibit very similar behaviour. The relatively slight cellular toxicity revealed by cationic lipids contribute to render these molecules very suitable for aODN vehiculation. Topics: 3T3 Cells; Adenocarcinoma; Animals; Base Sequence; Biological Transport; Cell Line; Colonic Neoplasms; Drug Resistance; Fatty Acids, Monounsaturated; Glycine; Humans; Kinetics; Leukemia; Mice; Molecular Sequence Data; Oligonucleotides, Antisense; Quaternary Ammonium Compounds; Spermine; T-Lymphocytes; Time Factors; Tumor Cells, Cultured	1993

Article

Year

Cationic lipids improve antisense oligonucleotide uptake and prevent degradation in cultured cells and in human serum.

Biochemical and biophysical research communications, 1993, Dec-15, Volume: 197, Issue:2

The power of antisense phosphodiester oligonucleotides (aODN) as regulatory molecules of gene expression is strongly limited by their low cellular uptake and very rapid nuclease-mediated degradation. This study deals with the effect of artificial cationic lipids on ODN cellular uptake and degradation in cell cultures and in human serum. At the ODN levels normally used in antisense-mediated gene regulation experiments, a cationic lipid, DOTAP, enhances the rate of ODN uptake more than 25 fold, but at lower ODN levels the effect of DOTAP is absent. These findings are consistent with a mechanism of ODN internalization by receptor-mediated saturable endocytosis that is bypassed by DOTAP. ODN degradation by nucleases is markedly prevented by DOTAP both in cultured cells and in human serum. Other cationic lipids, namely DOTMA and DOGS, exhibit very similar behaviour. The relatively slight cellular toxicity revealed by cationic lipids contribute to render these molecules very suitable for aODN vehiculation.

Topics: 3T3 Cells; Adenocarcinoma; Animals; Base Sequence; Biological Transport; Cell Line; Colonic Neoplasms; Drug Resistance; Fatty Acids, Monounsaturated; Glycine; Humans; Kinetics; Leukemia; Mice; Molecular Sequence Data; Oligonucleotides, Antisense; Quaternary Ammonium Compounds; Spermine; T-Lymphocytes; Time Factors; Tumor Cells, Cultured

1993