Compounds > n(4)-oleylcytosine-arabinoside

n(4)-oleylcytosine-arabinoside and Neoplasms

n(4)-oleylcytosine-arabinoside has been researched along with Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for n(4)-oleylcytosine-arabinoside and Neoplasms

Article	Year
Isolation and characterization of a scFv antibody specific for tumor endothelial marker 1 (TEM1), a new reagent for targeted tumor therapy. Cancer letters, 2006, Apr-28, Volume: 235, Issue:2 Tumor endothelial marker 1 (TEM1) is a protein predominantly expressed on the cell surface of endothelial cells in newly developing blood vessels and on tumor cells. It is therefore ideally suited as a target for anti-angiogenic tumor therapy. Using phage display technology a single chain antibody fragment (scFv-CM6) was isolated that specifically binds to the extracellular part of TEM1. Antibody specificity was determined in ELISA, by Western analysis, fluorescence microscopy and flow cytometry performed with TEM1-expressing cells. ScFv-CM6 was further functionalized and coupled to liposomes. Such immunoliposomes loaded with the cytotoxic drug N4-octadecyl-1-beta-D-arabinofuranosylcytosine-(5'-5')-3'-C-ethinylcytidine showed increased binding affinity and up to 80% higher cytotoxic activity towards TEM1-expressing IMR-32 tumor cells compared with control liposomes. Topics: Antibody Specificity; Antigens, CD; Antigens, Neoplasm; Cell Line; Cytarabine; Cytidine; Humans; Immunoglobulin Fragments; Liposomes; Membrane Proteins; Neoplasm Proteins; Neoplasms; Peptide Library; Recombinant Proteins	2006

Article

Year

Isolation and characterization of a scFv antibody specific for tumor endothelial marker 1 (TEM1), a new reagent for targeted tumor therapy.

Cancer letters, 2006, Apr-28, Volume: 235, Issue:2

Tumor endothelial marker 1 (TEM1) is a protein predominantly expressed on the cell surface of endothelial cells in newly developing blood vessels and on tumor cells. It is therefore ideally suited as a target for anti-angiogenic tumor therapy. Using phage display technology a single chain antibody fragment (scFv-CM6) was isolated that specifically binds to the extracellular part of TEM1. Antibody specificity was determined in ELISA, by Western analysis, fluorescence microscopy and flow cytometry performed with TEM1-expressing cells. ScFv-CM6 was further functionalized and coupled to liposomes. Such immunoliposomes loaded with the cytotoxic drug N4-octadecyl-1-beta-D-arabinofuranosylcytosine-(5'-5')-3'-C-ethinylcytidine showed increased binding affinity and up to 80% higher cytotoxic activity towards TEM1-expressing IMR-32 tumor cells compared with control liposomes.

Topics: Antibody Specificity; Antigens, CD; Antigens, Neoplasm; Cell Line; Cytarabine; Cytidine; Humans; Immunoglobulin Fragments; Liposomes; Membrane Proteins; Neoplasm Proteins; Neoplasms; Peptide Library; Recombinant Proteins

2006